Apr 18, 2020

Identification of functionally-distinct macrophage subpopulations regulated by efferocytosis in Drosophila

BioRxiv : the Preprint Server for Biology
J. A. CoatesIwan Robert Evans

Abstract

Macrophages are a highly heterogeneous population of cells, with this diversity stemming in part from the existence of tissue resident populations and an ability to adopt a variety of activation states in response to stimuli. Drosophila blood cells (hemocytes) are dominated by a lineage of cells considered to be the functional equivalents of mammalian macrophages (plasmatocytes). Until very recently plasmatocytes were thought to be a homogeneous population. Here, we identify enhancer elements that label subpopulations of plasmatocytes, which vary in abundance across the lifecourse of the fly. We demonstrate that these plasmatocyte subpopulations behave in a functionally-distinct manner when compared to the overall population, including more potent migratory responses to injury and decreased clearance of apoptotic cells within the developing embryo. Additionally, these subpopulations display differential localisation and dynamics in pupae and adults, hinting at the presence of tissue-resident macrophages in the fly. Our enhancer analysis also allows us to identify novel candidate genes involved in plasmatocyte behaviour in vivo. Misexpression of one such enhancer-linked gene (calnexin14D) in all plasmatocytes improves wound resp...Continue Reading

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Mentioned in this Paper

Embryo
Paucisalibacillus globulus
Mespilia globulus
Temnopleurus reevesii
Cell Motility
Extracellular Matrix
Etiology
Structure of Blastomere
Sea urchin (invertebrate)
Seashells

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