Morphological evaluation of vascular smooth muscle cell: length and width from a single scanning electron micrograph of microvessels

The Anatomical Record
B G MillerA P Evan

Abstract

Accurate three-dimensional data on the structure of vascular smooth muscle cells is essential for understanding the microvascular system in both normal or disease conditions. The laborious serial reconstruction methods have limited the amount of data collected on the structure of individual vascular smooth muscle (VSM) cells. The circumferential viewing of whole vessel segments via scanning electron microscopy provides an alternative approach, but even this technique is highly specialized and tedious. This study presents a simplified method to determine the average cell length and width of individual VSM cells by using only one view of a microvessel (single view). The vessels do not have to be isolated for circumferential viewing and can be left in their host tissue if desired. Values for the average VSM cell length and width were obtained by both circumferential- and single-view approach on the same vessels. The average cell length and width obtained from the single-view method (using one-third circumference) duplicated the mean length and width measurements obtained by circumferential viewing.

Citations

Jan 1, 1992·Electron Microscopy Reviews·H Nakamura, K Ohtsubo
Mar 2, 2013·Journal of Morphology·Sadaf Mumtaz
Oct 13, 2011·Basic & Clinical Pharmacology & Toxicology·Luis A Martinez-Lemus
Feb 18, 2017·Small·João RibasAli Khademhosseini
Feb 24, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luis A Martinez-LemusGerald A Meininger
Feb 9, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M A HillS Price
Oct 30, 2020·Journal of Applied Physiology·Maarten H G HeusinkveldWouter Huberts

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