PMID: 9179749May 1, 1997Paper

Morphological evidence of apoptosis in chickens infected with infectious bursal disease virus

Journal of Comparative Pathology
K M Lam

Abstract

Two-week-old specific pathogen-free chickens were infected with the infectious bursal disease virus by the ocular route. Immediately, and at 4 and 8 days after infection, groups of three chickens were killed and tissue samples were collected. Under electron microscopical examination, typical apoptotic cells were seen in the bursa of Fabricius (BF) and in the spleen. Tissue sections stained with haematoxylin and eosin were subjected to image analysis to quantify cellular depletion in the follicles of the BF. Unstained sections were treated with a terminal deoxy-transferase-based kit to detect apoptotic cells. The numbers of apoptotic cells at different stages of infection were counted by image analysis. The results revealed a rapid depletion of cells in the BF and a simultaneous increase in apoptotic cells.

References

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Citations

Sep 23, 2006·Virus Genes·Haisong LuAizhen Guo
Oct 18, 2003·Avian Diseases·Mary J Pantin-Jackwood, Thomas P Brown
Sep 29, 2005·Comparative Immunology, Microbiology and Infectious Diseases·Juan Carlos Rodríguez-LecompteFrederick S B Kibenge
Mar 16, 2018·Journal of Virology·Liliana L Cubas-GaonaDolores Rodríguez
Apr 28, 2017·Avian Pathology : Journal of the W.V.P.A·Marcelo Pablo KillianDaniel Marcelo Lombardo

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis