Morphological examination of bone synthesis via direct administration of basic fibroblast growth factor into rat bone marrow

Microscopy Research and Technique
Norio AmizukaH Ozawa

Abstract

Woven bone induced by direct injection of basic fibroblast growth factor (bFGF) into rat bone marrow was examined. On the first day after injection, fibrous tissues formed in the treated region of the bone marrow. Tissue-nonspecific alkaline phosphatase (TNAPase)-immunopositive osteoblastic cells and osteopontin immunopositive-extracellular matrices were observed in the fibrous tissues, indicating bone induction. On the fifth day, the bFGF-induced bone was found broadly in the bone marrow. In the originally existing bone, osteopontin-immunoreactivity was observed at cement lines, but not in the fully calcified matrix, whereas the woven bone displayed immunoreactivity throughout the matrix. Numerous TRAPase-positive osteoclasts were present on the surfaces of the woven bone, but no obvious cement line was observed. Therefore, both bone formation and resorption appeared highly active, without normal cellular coupling equilibrated between bone formation and resorption performed by osteoblasts and osteoclasts. On the tenth day, the bFGF-induced bone was almost replaced by bone marrow. Thus, bone formation actively occurred in the first half of the experimental period, whereas bone resorption came to be predominant thereafter. This ...Continue Reading

References

Aug 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T IkedaS Yoshiki
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F P ReinholtD Heinegård
Sep 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N TakahashiT Suda
Dec 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H A Simmons, L G Raisz
Oct 1, 1991·Acta Orthopaedica Scandinavica·P AspenbergL S Lohmander
Nov 1, 1988·Endocrinology·N TakahashiT Suda
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A OldbergD Heinegård
May 1, 1988·The Journal of Clinical Investigation·E CanalisT McCarthy
Feb 1, 1980·The Anatomical Record·A Vignery, R Baron
Aug 1, 1995·The Journal of Clinical Investigation·H KawaguchiM M Hurley
Aug 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M IwasakiK Ono
Oct 1, 1993·Acta Orthopaedica Scandinavica·J S Wang, P Aspenberg
Feb 1, 1993·Developmental Biology·K PetersL Williams
Mar 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L J SuvaG A Rodan
Apr 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M D McKeeA Nanci
Mar 1, 1997·Histochemistry and Cell Biology·K HoshiH Ozawa
Jun 1, 1961·The American Journal of Physiology·H FLEISH, W F NEUMAN
Jan 1, 1923·The Biochemical Journal·R Robison
May 1, 1995·Trends in Cardiovascular Medicine·C M GiachelliL Liaw

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Citations

Dec 6, 2008·Journal of the American Society of Nephrology : JASN·Hiroko SegawaKen-Ichi Miyamoto
Jan 22, 2009·The Tohoku Journal of Experimental Medicine·Reiko Takano-MurakamiNaoto Endo
Feb 6, 2008·Journal of Anatomy·Hironobu SuzukiTakeyasu Maeda
Oct 9, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Patricia Collin-OsdobyPhilip Osdoby
Aug 30, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Maiko HagaTakeyasu Maeda
Sep 15, 2006·Microscopy Research and Technique·Naoki KondoNaoto Endo
Oct 31, 2007·Journal of Bone and Mineral Metabolism·Satoshi HiroseNorio Amizuka
Sep 21, 2005·Microscopy Research and Technique·Kazi Sazzad HossainTakeyasu Maeda
Mar 1, 2006·Archives of Histology and Cytology·Hironobu SuzukiTakeyasu Maeda

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