Abstract
Bacterial biofilms can enhance survival in adverse environments and promote infection. However, little is known about biofilm formation by Clostridium perfringens. To better characterize this process, we used SEM to observe the surfaces of C. perfringens biofilms after 12, 24, 48, and 72 h of incubation. Biofilm cells appeared to be encased in a dense matrix material, and the total biomass of the biofilm increased with incubation time. To gain insight into the differentially expressed genes (DEGs) between biofilm and planktonic cells, we carried out comparative transcriptomic analysis using RNA sequencing. In total, 91 genes were significantly differentially expressed, with 40 being up-regulated and 51 down-regulated. In particular, genes encoding sortase, ribosomal proteins, and ATP synthase were up-regulated in biofilms, while genes coding for clostripain and phospholipase C were down-regulated. To validate the RNA sequencing results, qRT-PCR analysis was performed using five randomly selected DEGs. Results showed that all five genes were up-regulated, which was in accordance with the RNA sequencing results. To examine the functional differences, the DEGs were characterized by GO and KEGG pathway enrichment analyses. Results ...Continue Reading
References
Apr 22, 1999·Trends in Microbiology·L PetitM R Popoff
May 21, 1999·Science·J W CostertonE P Greenberg
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Feb 15, 2001·Trends in Microbiology·J W Costerton
Sep 29, 2001·Journal of Clinical Pathology·N Modi, M H Wilcox
Mar 6, 2003·Journal of Bacteriology·Nicola R StanleyBeth A Lazazzera
Jul 10, 2003·Microbes and Infection·Ing-Marie JonssonAndrzej Tarkowski
Jul 15, 2003·Clinical Microbiology Reviews·Amy E Bryant
Jul 23, 2003·Infection and Immunity·Keeta S GilmoreMichael S Gilmore
Jan 1, 2005·Spine·Kee-Yong HaSeung-Joon Ryoo
Sep 4, 2008·Infection and Immunity·John J VargaStephen B Melville
Oct 27, 2009·Bioinformatics·Likun WangXuegong Zhang
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
Feb 13, 2010·International Journal of Antimicrobial Agents·Niels HøibyOana Ciofu
Jan 18, 2011·Microbial Pathogenesis·Nobuo OkahashiTakashi Ooshima
May 17, 2011·Journal of Bacteriology·Renee M SavilleAlfred M Spormann
Jun 24, 2011·Molecular Microbiology·Arunima MishraHung Ton-That
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
May 11, 2013·Foodborne Pathogens and Disease·M Kate ThomasBarbara Marshall
Feb 11, 2014·Journal of Bacteriology·Nozomu ObanaNobuhiko Nomura
Mar 19, 2014·Microbiology·Shota SuzukiYasuhiko Sekine
May 6, 2014·Frontiers in Microbiology·Audrey CharleboisMarie Archambault
Apr 1, 2015·Infection and Immunity·Jorge E VidalAdrian Canizalez-Roman
Oct 18, 2015·RNA·Cristina MaracciMarina V Rodnina
May 22, 2016·Avian Pathology : Journal of the W.V.P.A·Audrey CharleboisMarie Archambault
Jan 1, 2013·Burns and Trauma·Daniel G Metcalf, Philip G Bowler
Nov 28, 2016·Food Microbiology·Audrey CharleboisMarie Archambault
Jul 8, 2017·Bioorganic & Medicinal Chemistry·Lena SchmohlDirk Schwarzer