Morphological phenotyping of enteric neurons using neurofilament immunohistochemistry renders chemical phenotyping more precise in porcine ileum

Histochemistry and Cell Biology
Axel BrehmerWinfried Neuhuber

Abstract

The aim of the study was: (1) to test the suitability of neurofilament (NF) immunohistochemistry for representing the shapes of morphologically defined neuron types in the pig ileum myenteric plexus, (2) to estimate the proportions of these neuron types as related to the whole myenteric neuron population and (3) to demonstrate the usefulness of a refined morphological classification of enteric neurons on the paradigm of calcitonin gene-related peptide (CGRP)-immunoreactive neurons. So far, immunoreactivity for this peptide was supposed to be present in the pig enteric nervous system only in type II neurons. Ileal whole mounts of two pigs were stained with the cuprolinic blue (CB) method and, thereafter, incubated with an antibody pool against NF proteins (70, 160 and 210 kDa), visualised with a fluorochrome-tagged secondary antibody. The structural representation of morphologically defined myenteric neuron types typical for pig ileum (Stach I, II, IV, V and VI) was equivalent to their silver impregnated image, as demonstrated in previous studies. Counts of CB-stained neurons revealed between 2,526 and 2,662 neurons per square centimetre in one pig and between 2,027 and 2,763 in the other. As related to these total neuron number...Continue Reading

Citations

Sep 21, 2007·Histochemistry and Cell Biology·Simone WeidmannAxel Brehmer
Oct 16, 2008·Cell and Tissue Research·Zheng-Dong QuJohn B Furness
Nov 15, 2003·Autonomic Neuroscience : Basic & Clinical·Marion RaabWinfried L Neuhuber
Oct 3, 2002·The Journal of Comparative Neurology·Axel BrehmerWinfried Neuhuber
Sep 5, 2012·Anatomia, histologia, embryologia·J SadeghinezhadR Chiocchetti
Jul 6, 2004·Autonomic Neuroscience : Basic & Clinical·Axel BrehmerWinfried Neuhuber
Dec 28, 2006·Cells, Tissues, Organs·Sonja KappAxel Brehmer
Jan 7, 2009·Acta histochemica·Catharina Olsson
Sep 1, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Matthias WolfAxel Brehmer
Dec 17, 2002·Histochemistry and Cell Biology·Esther Asan
Jun 26, 2021·Histochemistry and Cell Biology·Axel Brehmer

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