Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia.

Parasitology Research
Viera NagyováJozef Timko

Abstract

Acanthamoeba keratitis (AK) is a relatively rare disease worldwide. Over the past 10 years, five cases of AK were reported in Slovakia. Four preserved Slovak strains and one strain from the Czech Republic isolated from corneal scrapes of patients with AK are characterised in this study. Genotype identification of isolates is based on sequences of the PCR amplimer GTSA.B1 amplified from 18S ribosomal DNA. A strain isolated from the first patient in 1999 was classified as a rare sequence type T15. This is just the second report in which genotype T15 has been associated with AK. The other three Slovak strains were identified as belonging to the most common genotype T4. The only strain originating from the Czech Republic was classified as sporadically appearing sequence type T3. All isolates were also studied for their temperature tolerance and growth characteristics. The cythopatic effect was tested in vitro on Vero cell cultures.

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Citations

May 27, 2014·Parasitology Research·A MagnetC del Aguila
Nov 20, 2015·Parasitology Research·Maritza Omaña-MolinaAdolfo Martinez-Palomo
Jul 23, 2014·The Journal of Eukaryotic Microbiology·Julia WalochnikEva-Maria Haller-Schober
Jan 5, 2011·Acta Tropica·Karin Caumo, Marilise B Rott
Jul 24, 2012·Diagnostic Microbiology and Infectious Disease·Pablo GoldschmidtChristine Chaumeil
Jan 22, 2015·Parasitology Research·Monika DerdaEdward Hadaś
Apr 15, 2016·Acta Parasitologica·Laura Fuhrich FabresMarilise Brittes Rott
Feb 13, 2017·Parasitology Research·Daniele CorsaroJulia Walochnik
Aug 6, 2021·Parasitology Research·Maria Luisa Nunes DiehlMarilise Brittes Rott
Nov 16, 2021·PloS One·Faten A M HassanSara S Abdel-Hakeem

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