Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis.

Journal of Industrial Microbiology & Biotechnology
D J BarryG A Williams

Abstract

Mycelial morphology is a critically important process property in industrial fermentations of filamentous micro-organisms, as particular phenotypes are associated with maximum productivity. However, the accurate quantification of complex morphologies still represents a significant challenge in elucidating this relationship. A system has been developed for high-resolution characterisation of filamentous fungal growth on a solid substrate, using membrane immobilization and fully-automatic plug-ins developed for the public domain, Java-based, image-processing software, ImageJ. The system has been used to quantify the microscopic development of Aspergillus oryzae on malt agar, by measuring spore projected area and circularity, the total length of a hyphal element, the number of tips per element, and the hyphal growth unit. Two different stages of growth are described, from the swelling of a population of conidiospores up to fully developed, branched hyphae 24 h after inoculation. Spore swelling expressed as an increase in mean equivalent spore diameter was found to be approximately linear with time. Widespread germination of spores was observed by 8 h after inoculation. From approximately 12 h, the number of tips was found to incre...Continue Reading

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Citations

Nov 28, 2012·Trends in Biotechnology·Andreas E PoschOliver Spadiut
May 17, 2012·Fungal Genetics and Biology : FG & B·Andreas E PoschChristoph Herwig
Apr 14, 2015·Biotechnology Progress·David J BarryCecilia Chan
Jul 10, 2012·Journal of Biotechnology·Rainer KrullChristoph Wittmann
Feb 16, 2019·Biotechnology and Bioengineering·Stefan SchmidederHeiko Briesen
Sep 6, 2012·Biotechnology and Bioengineering·David J Barry
May 20, 2017·Microbiology Spectrum·Mark D FrickerLynne Boddy

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