Morphological, toxicological and molecular characterization of a benthic Nodularia isolated from Atlantic estuarine environments

Research in Microbiology
Viviana R LopesVitor Vasconcelos

Abstract

A polyphasic study of a benthic Nodularia isolate (LEGE06071) from an Atlantic environment, specifically salt pans, was performed. LEGE06071 resembled both type strains of Nodularia sphaerocarpa and Nodularia harveyana, while ACOI00729 (purchased isolate) was identified as N. sphaerocarpa. The length and width of vegetative cells varied from 3.10 to 3.15 microm and from 3.71 to 4.25 microm, respectively, while heterocyts were 3.91-4.89 microm long and 4.20-4.74 microm wide. None of the isolates had aerotopes. The sequencing of the 16S rRNA gene from the two Nodularia isolates indicated that they belonged neither to Nodularia spumigena nor N. harveyana. Nodularin and other cyanotoxin synthesis-associated genes could not be detected, nor could nodularin production be detected by ELISA. However, MALDI-TOF analysis of LEGE06071 revealed the presence of other compounds, namely, glycolipids. Hence, toxicological screenings against Artemia nauplii, Escherichia coli and Salmonella typhimurium were performed. Toxic effects could only be observed against Artemia, with 48 h-LC(50) values for the aqueous and crude extract of methanol of 53.21 mg ml(-1) and 17.81 mg ml(-1), respectively. This study presents the first evidence of a non-nodul...Continue Reading

References

Jan 1, 1992·Mutation Research·V Mersch-SundermannS Kevekordes
Apr 1, 1968·Journal of General Microbiology·M M Allen, R Y Stanier
Jul 1, 1997·International Journal of Systematic Bacteriology·B A NeilanA E Goodman
Jan 27, 1999·Bioinformatics·D Posada, K A Crandall
Jun 8, 2000·International Journal of Systematic and Evolutionary Microbiology·J LehtimäkiK Sivonen
Dec 13, 2000·Mutation Research·K Mortelmans, E Zeiger
Feb 15, 2001·Journal of Natural Products·K SaitoC Chen
Jun 8, 2001·Trends in Pharmacological Sciences·J P GrotenJ Sühnel
Sep 26, 2001·Applied and Environmental Microbiology·M J LaamanenK Sivonen
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Oct 11, 2003·Archives of Microbiology·Michael HisberguesThomas Börner
Oct 16, 2003·Toxicon : Official Journal of the International Society on Toxinology·Gary S CaldwellPeter J W Olive
Mar 2, 2005·Toxicology and Applied Pharmacology·C Wiegand, S Pflugmacher
Mar 19, 2005·International Journal of Systematic and Evolutionary Microbiology·Christina LyraKaarina Sivonen
Apr 26, 2007·Journal of Industrial Microbiology & Biotechnology·Martin E Barrios-LlerenaPhillip C Wright
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 10, 2007·Toxicon : Official Journal of the International Society on Toxinology·Rosário MartinsVitor Vasconcelos
Nov 18, 2008·Critical Reviews in Toxicology·L A PearsonNeilan B A

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.