Morphologically identified cutaneous afferent DRG neurons express three different potassium currents in varying proportions

Journal of Neurophysiology
B EverillJ D Kocsis

Abstract

Outward K+ currents were recorded using a whole cell patch-clamp configuration, from acutely dissociated adult rat cutaneous afferent dorsal root ganglion (DRG) neurons (L4 and L5) identified by retrograde labeling with Fluoro-gold. Recordings were obtained 16-24 h after dissociation from cells between 39 and 49 mm in diameter with minimal processes. These cells represent medium-sized DRG neurons relative to the entire population, but are large cutaneous afferent neurons giving rise to myelinated axons. Voltage-activated K+ currents were recorded routinely during 300-ms depolarizing test pulses increasing in 10-mV steps from -40 to +50 mV; the currents were preceded by a 500-ms conditioning prepulse of either -120 or -40 mV. Coexpression of at least three components of K+ current was revealed. Separation of these components was achieved on the basis of sensitivities to the K+ channel blockers, 4-aminopyridine (4-AP) and dendrotoxin (DTx), and by the current responses to variation in conditioning voltage. Changing extracellular K+ concentration from 3 to 40 mM resulted in a shift to the right of the I-V curve commensurate with K+ being the principal charge carrier. Presentation of 100 mM 4-AP revealed a rapidly activating K+ cur...Continue Reading

References

Feb 1, 1977·The Journal of Physiology·S H Thompson
Oct 2, 1992·Brain Research·J M CaffreyJ D Kocsis
Oct 1, 1992·Physiological Reviews·B S Pallotta, P K Wagoner
Jan 1, 1992·The Journal of Physiology·E Ficker, U Heinemann
Oct 1, 1991·Journal of Neurophysiology·S McFarlane, E Cooper
Dec 1, 1990·Journal of Neurophysiology·M Devor, P D Wall
Jan 1, 1990·Pharmacology & Therapeutics·P N Strong
Jan 1, 1990·Progress in Brain Research·J F Storm
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J V HalliwellJ O Dolly
Oct 1, 1986·Pflügers Archiv : European journal of physiology·R PennerF Dreyer
Dec 1, 1987·The Journal of Physiology·R E NumannR K Wong
Jun 1, 1988·Neuroscience·B Rudy
Oct 1, 1988·The Journal of Membrane Biology·E MoczydlowskiA Ravindran
Feb 1, 1989·Trends in Neurosciences·N A CastleD H Jenkinson
May 1, 1988·Trends in Neurosciences·J O Dolly
Jul 1, 1989·The Journal of Physiology·S G Cull-CandyD Ogden
Dec 1, 1988·Journal of Neurophysiology·D L EngS G Waxman
Oct 31, 1988·Neuroscience Letters·C Stansfeld, A Feltz
Feb 1, 1971·The Journal of Physiology·J A Connor, C F Stevens
Feb 1, 1971·The Journal of Physiology·J A Connor, C F Stevens
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jun 1, 1984·Journal of Neurophysiology·M Segal, J L Barker

❮ Previous
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Citations

Feb 15, 2002·Brain : a Journal of Neurology·Ron AmirMarshall Devor
Apr 29, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Guang-Yin XuPankaj Jay Pasricha
Jun 26, 2012·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Patrick L Stemkowski, Peter A Smith
Mar 10, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Rafael Taeho HanHeung Sik Na
Aug 22, 2006·European Journal of Pain : EJP·Forrest L Smith, Raymond J Lindsay
Oct 30, 2014·Neuromodulation : Journal of the International Neuromodulation Society·Elliot S Krames
Aug 19, 2003·The Journal of Physiology·Timothy StewartStephen Vanner
Apr 16, 2005·The Journal of Pharmacology and Experimental Therapeutics·Shinki Yoshida, Shigeji Matsumoto
Oct 4, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·K DangG F Gebhart
Apr 17, 2014·Antioxidants & Redox Signaling·Nikita Gamper, Lezanne Ooi
Aug 22, 2018·Frontiers in Molecular Neuroscience·Benjamin M ZemelTanziyah Muqeem
Feb 24, 2006·Journal of Neuroscience Research·Lin PiaoSeog Bae Oh
Sep 24, 2005·The European Journal of Neuroscience·Cécile HilaireFrédérique Scamps
Jun 6, 2009·The American Journal of Gastroenterology·Ai-Hua QianYao-Zong Yuan
Mar 13, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yukio HayashiNaoki Yoshimura
Dec 22, 1999·Journal of Neurophysiology·A Bordey, H Sontheimer
Apr 20, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jiangru HongGuangqin Zhang
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·M N RasbandJ S Trimmer
Oct 24, 2020·Frontiers in Cellular Neuroscience·Peter A Smith
Oct 17, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Cosmin I Ciotu, Michael J M Fischer

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