Morphologically stable epithelial vesicles cultured from normal human endometrium in defined media

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
D Kirk, R B Alvarez

Abstract

Epithelium from normal human endometrium was cultured as morphologically stable vesicular structures in a defined, hormone-supplemented PFMR-4 medium. The structures consisted of a single layer of polarized epithelial cells with the apical surface facing the external culture medium, and the basal surface resting on a well-defined basal lamina adjacent to the internal lumen. Vesicles were shown to retain their viability for up to 3 mo. in culture, to actively synthesize DNA after being cultured for over a month in a defined medium, and to respond to steroid hormones. When embedded within a collagen gel, the vesicles reversed their epithelial polarity and formed branching, pseudoglandular structures. It was concluded that the three-dimensional shape of the epithelial vesicles had a critical role to play in their morphological stability, nutrient requirement, and hormone sensitivity.

References

Apr 1, 1979·The Journal of Clinical Endocrinology and Metabolism·P G SatyaswaroopE Gurpide
Jan 1, 1983·International Review of Cytology·J Yang, S Nandi
Feb 1, 1980·American Journal of Obstetrics and Gynecology·J M TrentC M Payne
Oct 1, 1980·Wilhelm Roux's Archives of Developmental Biology·F M A van Breugel, C Grond

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Citations

Jul 1, 1990·Cell and Tissue Research·L MunsonD H Schlafer
Oct 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C A RinehartD G Kaufman
Aug 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S GithensC L Patke
Jan 1, 1992·Pharmacology & Therapeutics·U Bjare
Apr 30, 2020·Tissue Engineering. Part a·Hannes CampoJ Julie Kim
Oct 4, 2020·Biology of Reproduction·Harriet C FitzgeraldThomas E Spencer
Feb 4, 2021·Reproductive Sciences·Yong Song, Asgerally T Fazleabas

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