Morphology and Function of the Lamb Ileum following Preterm Birth

Frontiers in Pediatrics
Tracey J FloresMary Jane Black

Abstract

For infants born moderately/late preterm (32-37 weeks of gestation), immaturity of the intestine has the potential to impact both short- and long-term gastrointestinal function. The aim of this study conducted in sheep was to compare the morphology and smooth muscle contractility of the ileum in term and late preterm lambs. Lambs delivered preterm (132 days gestation;n = 7) or term (147 days gestation;n = 9) were milk-fed after birth and euthanased at 2 days of age. A segment of distal ileum was collected for analysis of the length and cellular composition of the villi and crypts, smooth muscle width and contractility, and mRNA expression of the cell markers Ki67, lysozyme, mucin 2, synaptophysin, chromogranin A, olfactomedin 4, axis inhibition protein 2, and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5). There was no difference in the proportion of inflammatory, proliferating, apoptotic, enterocyte, or goblet cells between groups, but preterm lambs exhibited a significant upregulation of the stem cell marker LGR5 (p = 0.01). Absolute villus height (term: 1,032 ± 147 µm, preterm: 651 ± 52 µm;p < 0.0001) and crypt depth (term: 153 ± 11 µm, preterm: 133 ± 17 µm;p = 0.01) were significantly shorter in the pret...Continue Reading

References

May 1, 1989·Journal of Pediatric Gastroenterology and Nutrition·C G AvilaP M Robinson
Mar 1, 1986·The Anatomical Record·J Trahair, P Robinson
Mar 1, 1984·Archives of Disease in Childhood·L T WeaverR Nelson
Sep 14, 1999·JPEN. Journal of Parenteral and Enteral Nutrition·A Lebenthal, E Lebenthal
Jan 10, 2003·The Journal of Pediatrics·Katsumi Mizuno, Aki Ueda
Oct 31, 2003·American Journal of Obstetrics and Gynecology·Noboru OyachiMichael G Ross
Apr 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Antoni StadnickiRobert W Colman
Jun 14, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·M DevaniM Cugno
Feb 8, 2007·The American Journal of Clinical Nutrition·Josef Neu
Nov 17, 2007·Cardiovascular Research·Antony VinhTracey A Gaspari
Dec 7, 2007·Pediatrics·William A EngleUNKNOWN Committee on Fetus and Newborn, American Academy of Pediatrics
Jan 8, 2008·Lancet·Robert L GoldenbergRoberto Romero
Aug 1, 2008·Gut·M CostaR Sjodahl
May 13, 2009·Journal of Pediatric Surgery·Marie-Chantal StruijsPaul W Wales
Sep 18, 2010·Inflammatory Bowel Diseases·Antoni Stadnicki
Jan 21, 2011·The New England Journal of Medicine·Josef Neu, W Allan Walker
Sep 29, 2011·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Flavia IndrioRuggiero Francavilla
Oct 19, 2011·Infection and Immunity·Nadine R Martinez RodriguezEdith Porter
Dec 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kelley S YanCalvin J Kuo
Jun 19, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Lei WangJishu Shi
Jan 23, 2016·Nature·Michael Karin, Hans Clevers
Jun 9, 2016·Scientific Reports·Yongyan ShiYan Chun Li
Apr 27, 2017·Lancet·Jane E HardingFrank H Bloomfield

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Citations

Jun 6, 2020·Scientific Reports·Clara Yieh Lin ChongJustin M O'Sullivan

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Methods Mentioned

BETA
periodic acid Schiff

Software Mentioned

GraphPad Prism
Graphpad
Image Pro - Plus

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