Morphology-Driven Control of Metabolite Selectivity Using Nanostructure-Initiator Mass Spectrometry

Analytical Chemistry
Jian GaoTrent R Northen

Abstract

Nanostructure-initiator mass spectrometry (NIMS) is a laser desorption/ionization analysis technique based on the vaporization of a nanostructure-trapped liquid "initiator" phase. Here we report an intriguing relationship between NIMS surface morphology and analyte selectivity. Scanning electron microscopy and spectroscopic ellipsometry were used to characterize the surface morphologies of a series of NIMS substrates generated by anodic electrochemical etching. Mass spectrometry imaging was applied to compare NIMS sensitivity of these various surfaces toward the analysis of diverse analytes. The porosity of NIMS surfaces was found to increase linearly with etching time where the pore size ranged from 4 to 12 nm with corresponding porosities estimated to be 7-70%. Surface morphology was found to significantly and selectively alter NIMS sensitivity. The small molecule (<2k Da) sensitivity was found to increase with increased porosity, whereas low porosity had the highest sensitivity for the largest molecules examined. Estimation of molecular sizes showed that this transition occurs when the pore size is <3× the maximum of molecular dimensions. While the origins of selectivity are unclear, increased signal from small molecules wit...Continue Reading

References

Feb 13, 2003·Chemical Reviews·Klaus Dreisewerd
Feb 13, 2003·Chemical Reviews·Michael Karas, Ralf Krüger
Dec 9, 2003·Journal of Dentistry·C LouwerseM C D N J M Huysmans
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Chensong PanBaochuan Guo
Sep 21, 2007·Journal of the American Society for Mass Spectrometry·Trent R NorthenGary Siuzdak
Oct 26, 2007·Nature·Trent R NorthenGary Siuzdak
Jan 5, 2010·Metabolomics : Official Journal of the Metabolomic Society·Augustin ScalbertSuzan Wopereis
Apr 24, 2010·Analytical Chemistry·Rachel D LoweNicolas H Voelcker
Aug 31, 2010·Analytical Chemistry·Ranu Nayak, Daniel R Knapp
Mar 13, 2012·Analytical and Bioanalytical Chemistry·Matthew GrevingTrent Northen
Mar 15, 2012·Physical Chemistry Chemical Physics : PCCP·Jessica A StoleeAkos Vertes
Jul 24, 2012·Nanoscale Research Letters·Hyohan Kim, Namhee Cho
Oct 4, 2013·Analytical Chemistry·Oliver RübelBenjamin P Bowen
Jun 12, 2014·Mass Spectrometry Reviews·Taryn GuinanNicolas H Voelcker
Jan 8, 2016·Analytical Chemistry·Jian GaoTrent R Northen
Jan 21, 2017·ACS Applied Materials & Interfaces·Hazem H AbdelmaksoudNicolas H Voelcker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.