PMID: 9436999Mar 14, 1998Paper

Morphology of the yeast endocytic pathway

Molecular Biology of the Cell
C Prescianotto-Baschong, H Riezman

Abstract

Positively charged Nanogold (Nanoprobes, Stony Brook, NY) has been developed as a new marker to follow the endocytic pathway in yeast. Positively charged Nanogold binds extensively to the surface of yeast spheroplasts and is internalized in an energy-dependent manner. Internalization of gold is blocked in the end3 mutant. During a time course of incubation of yeast spheroplasts with positively charged Nanogold at 15 degrees C, the gold was detected sequentially in small vesicles, a peripheral, vesicular/tubular compartment that we designate as an early endosome, a multivesicular body corresponding to the late endosome near the vacuole, and in the vacuole. Experiments examining endocytosis in the sec18 mutant showed an accumulation of positively charged Nanogold in approximately 30-50 nm diameter vesicles. These vesicles most likely represent the primary endocytic vesicles as no other intermediates were detected in the mutant cells, and they correspond in size to the first vesicles detected in wild-type spheroplasts at 15 degrees C. These data lend strong support to the idea that the internalization step of endocytosis in yeast involves formation of small vesicles of uniform size from the plasma membrane.

References

Mar 1, 1991·The Journal of Cell Biology·P G Woodman, G Warren
Mar 8, 1991·Cell·J P GorvelJ Gruenberg
Jan 1, 1991·Methods in Enzymology·V DulicH Riezman
Mar 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E van Tuinen, H Riezman
Mar 1, 1995·The Journal of Cell Biology·T A Vida, S D Emr
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·W T WongP P Di Fiore
Nov 1, 1994·Molecular and Cellular Biology·K A Schandel, D D Jenness
Dec 1, 1994·The Journal of Cell Biology·R G PartonK Simons
Jul 1, 1993·The Journal of Cell Biology·N G DavisG F Sprague
Dec 1, 1995·The Journal of Cell Biology·A BenmerahN Cerf-Bensussan
Jan 1, 1996·The Journal of Cell Biology·W StoorvogelH J Geuze
Nov 15, 1996·The Journal of Biological Chemistry·F TebarT Kirchhausen
Dec 15, 1996·Experientia·H RiezmanL Hicke
Aug 15, 1997·The Journal of Biological Chemistry·C LamazeS L Schmid
Jul 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jürgen GentGer J Strous
Aug 1, 1993·Trends in Cell Biology·H Riezman

❮ Previous
Next ❯

Citations

Feb 13, 2001·Microscopy Research and Technique·J Vorísek
Feb 13, 2001·Microscopy Research and Technique·A L Munn
Nov 7, 2008·Protoplasma·M Götte, T Lazar
Oct 19, 2005·Protoplasma·U Fuchs, G Steinberg
Aug 28, 1998·Current Opinion in Cell Biology·B WendlandH Riezman
Nov 15, 2002·Fungal Genetics and Biology : FG & B·Sara Torralba, I Brent Heath
May 15, 2001·Traffic·J J Baggett, B Wendland
Apr 16, 2002·Molecular Biology of the Cell·Eva M NeuhausThierry Soldati
Nov 14, 2002·Molecular Biology of the Cell·Fatima-Zahra IdrissiM Isabel Geli
Mar 9, 1999·Molecular Biology of the Cell·J MulhollandD Botstein
Jan 19, 2000·Molecular Biology of the Cell·M J LewisH R Pelham
Oct 12, 2000·Molecular Biology of the Cell·S GurunathanJ E Gerst
Feb 26, 2000·Molecular Biology of the Cell·S R GerrardT H Stevens
Dec 8, 1998·Molecular Biology of the Cell·J C HolthuisH R Pelham
Nov 5, 2004·Molecular Biology of the Cell·Avital A RodalJohn H Hartwig
Jan 6, 2006·Molecular Biology of the Cell·Kyoungtae KimJohn A Cooper
Oct 6, 2006·Molecular Biology of the Cell·Aki Yoneda, Tamara L Doering
Oct 16, 2009·Molecular Biology of the Cell·Daniel F MarkgrafChristian Ungermann
Mar 23, 2012·Molecular Biology of the Cell·Natasha S SavageDaniel J Lew
Jul 8, 2008·Journal of Experimental Botany·Elisabetta OnelliAlessandra Moscatelli
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·James B Moseley, Bruce L Goode
May 14, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Janice Griffith, Fulvio Reggiori
Sep 5, 2013·Cellular and Molecular Life Sciences : CMLS·Fatima-Zahra Idrissi, María Isabel Geli
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·S SpringerR Schekman
Oct 8, 1998·Molecular Biology of the Cell·K SéronR Haguenauer-Tsapis
Mar 22, 2002·Cellular Microbiology·Bruno RobibaroKeith A Joiner
Nov 30, 2005·The Journal of Cell Biology·Huaqing CaiSusan Ferro-Novick
Aug 19, 2009·Journal of Integrative Plant Biology·Alessandra Moscatelli, Aurora Irene Idilli
Sep 5, 2006·Trends in Cell Biology·F Gisou van der Goot, Jean Gruenberg
Apr 17, 2008·Journal of Structural Biology·Zhong-Ying ShenLi-Jun Tan
Feb 7, 2015·Molecular Biology of the Cell·Henning ArltChristian Ungermann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.