Morphometric Analysis of Hippocampal and Neocortical Pyramidal Neurons in a Mouse Model of Late Onset Alzheimer's Disease.

Journal of Alzheimer's Disease : JAD
Rasha H MehderR David Andrew

Abstract

The study of late-onset (sporadic) Alzheimer's disease (LOAD) has lacked animal models where impairments develop with aging. Oxidative stress promotes LOAD, so we have developed an oxidative stress-based model of age-related cognitive impairment based on gene deletion of aldehyde dehydrogenase 2 (ALDH2). This enzyme is important for the detoxification of endogenous aldehydes arising from lipid peroxidation. Compared to wildtype (WT) mice, the knockout (KO) mice exhibit a progressive decline in recognition and spatial memory and AD-like pathologies. Here we performed morphometric analyses in the dorsal and ventral hippocampal CA1 regions (dCA1 and vCA1) as well as in overlying primary sensory cortex to determine if altered neuronal structure can help account for the cognitive impairment in 12-month old KO mice. Dendritic morphology was quantitatively analyzed following Golgi-Cox staining using 9 WT mice (108 neurons) and 15 KO mice (180 neurons). Four pyramidal neurons were traced per mouse in each region, followed by branched structured analysis and Sholl analysis. Compared to WT controls, the morphology and complexity of dCA1 pyramidal neurons from KOs showed significant reductions in apical and basal dendritic length, dendrit...Continue Reading

References

Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·M B MoserR G Morris
Jan 9, 1999·Hippocampus·M B Moser, E I Moser
Aug 7, 2001·Journal of Neuropathology and Experimental Neurology·A NunomuraM A Smith
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kirsten G KjelstrupMay-Britt Moser
Nov 20, 2002·Pharmacogenetics·Toyohi IsseToshihiro Kawamoto
Dec 27, 2008·Neuron·Carol L ThompsonEd S Lein
Jun 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hong-Wei DongArthur W Toga
Feb 16, 2010·Neuron·Michael S Fanselow, Hong-Wei Dong
Mar 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Claudia Perez-CruzUlrich Ebert
Aug 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Saturnino SpigaMarco Diana
Sep 23, 2014·Nature Reviews. Neuroscience·Bryan A StrangeEdvard I Moser
Dec 3, 2014·Biochimica Et Biophysica Acta·Ginger L MilneL Jackson Roberts
Oct 21, 2015·Redox Biology·D Neil Granger, Peter R Kvietys
Jun 9, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bruce A BerkowitzRobin Roberts
Apr 17, 2018·Neurobiology of Learning and Memory·Ivan SkelinBruce L McNaughton

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
RNA-seq
gene targeting knockout
genotyping
PCR

Software Mentioned

Neurolucida System
Neurolucida Explorer
GraphPad Prism

Related Concepts

Related Feeds

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Alzheimer's Disease: Animal Models

Alzheimer's disease is a neurodegenerative disease which can be studied using various experimental systems. This feed focuses on animal models used for Alzheimer's disease research.