Morphometric and molecular differentiation of Phlebotomus (Phlebotomus) sandflies

Medical and Veterinary Entomology
N KhalidF Tripet

Abstract

The closely related sandfly species of the subgenus Phlebotomus namely, Phlebotomus papatasi (Scopoli, 1786), Phlebotomus duboscqi Neveu-Lemair, 1906 and Phlebotomus bergeroti Parrot, 1934 (Diptera: Psychodidae), are major vectors of Leishmania major (Kinetoplastida: Trypanosomatidae), the causative agent of cutaneous leishmaniasis in the Old World. Although allopatric in most of their distribution, the three species exist sympatrically in many places in central and eastern Sudan. Males of the three species can be distinguished using morphological characters; however, females are much harder to identify, thus complicating epidemiological studies. We carried out a morphometric and a molecular study to determine reliable morphological features and develop a polymerase chain reaction (PCR) assay for distinguishing females of these species. Males and females from each species were collected from sites in Sudan, East Africa and from one site in Mali, West Africa. Males were analysed morphologically and 20 characters and 10 character ratios were used in a stepwise discriminant analysis. This led to the identification of four characters with high discriminant loading scores sufficient for accurate male species identification. Male DNA...Continue Reading

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Citations

Aug 15, 2015·Bulletin of Entomological Research·B AltenJ M Medlock
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jérôme Depaquit
Jan 14, 2020·Pakistan Journal of Biological Sciences : PJBS·Mo Awia Mukhtar HassanSally O Widaa
Sep 21, 2018·BMC Veterinary Research·Valentina Foglia ManzilloGaetano Oliva

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