Morphostructural analysis of human follicular stem cells on highly porous bone hydroxyapatite scaffold

International Journal of Immunopathology and Pharmacology
S TetèR Ciccarelli

Abstract

In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3-6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.

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Citations

Apr 23, 2009·Neurotoxicity Research·M L CastellaniT C Theoharides
Dec 26, 2013·In Vitro Cellular & Developmental Biology. Animal·Weimin ZhuYanjun Zeng
Sep 15, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Javier Zurriaga CardaJosé L Gómez Ribelles

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