Morphotypic and genotypic characterization of sequential Candida parapsilosis isolates from an outbreak in a pediatric intensive care unit

Diagnostic Microbiology and Infectious Disease
Lucía García San MiguelSantiago Moreno

Abstract

Candidemia outbreaks that due to cross-infection are an emerging problem in hospitals. Typing of microorganisms is an essential tool for understanding the epidemiologic aspects of the infection. Techniques based on phenotypic characteristics are inexpensive and easy to perform but are limited by their lack of reproducibility. This study assessed the value of several phenotypic and genotypic techniques that are used in epidemiologic investigations of Candida parapsilosis in clinical practice and used a combination of these methods to analyze outbreak of C. parapsilosis candidemia. Random amplification of polymorphic DNA polymerase chain reaction with several primers was unsatisfactory because it lacked discriminatory power. By simplifying the reading of the morphotypes, we increased their reproducibility for each malt agar and 2,3,5-triphenyltetrazolium media (97% and 90%) and thus their suitability for its use. The combination of electrophoretic karyotype and the simplified morphotypes was rapid and practical to characterize the different clusters involved in the intensive care unit outbreak.

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Citations

Aug 8, 2009·Current Genetics·Jozef NosekLubomir Tomaska
May 31, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G PulcranoM R Catania
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Mar 21, 2006·Diagnostic Microbiology and Infectious Disease·Lucía García San MiguelSantiago Moreno
Jun 14, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R SabinoC Pais
Jun 7, 2006·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Lucia Garcia-San MiguelSantiago Moreno
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Nov 22, 2008·Scandinavian Journal of Infectious Diseases·Anna Brillowska-DabrowskaM C Arendrup
Feb 17, 2021·The Pediatric Infectious Disease Journal·Eloise J SilvesterAmanda Gwee

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