Morulin Pm: a modified polypeptide containing TOPA and 6-bromotryptophan from the morula cells of the ascidian, Phallusia mammillata

Archives of Biochemistry and Biophysics
S W TaylorM de Vincentiis

Abstract

A novel polypeptide containing the unusual posttranslationally modified amino acids L-3,4,5-trihydroxyphenylalanine (TOPA) and L-6-bromotryptophan (6-BrW) has been isolated from the morula cells of the vanadium-accumulating ascidian, Phallusia mammillata. The polypeptide, designated Morulin Pm, has a molecular weight of 3825 +/- 0.6 and has a simple amino acid composition consisting mainly of TOPA and 6-BrW as well as Ser, Leu, Phe, and Ala. To our knowledge, this is the first reported example of multiple sites of brominated tryptophan in a polypeptide of this size. Edman degradation revealed the N-terminal sequence to be BrW-Leu-Phe-BrW before sequencing was blocked. While the N-terminal tripeptide could be isolated from chymotrypsin digests of Morulin Pm, the rest of the polypeptide resisted further cleavage by the proteases, a feature common among this class of peptides. However, unlike other ascidian blood cell peptides examined to date, microheterogeneity was minimal. For the first time a detailed NMR investigation could be undertaken on a member of this class of polypeptides. In addition to signals assignable to the constituent amino acids by extensive 2D experiments, resonances were present both in the 13C and 1H spectra...Continue Reading

Citations

Feb 23, 2011·Marine Drugs·Manickam Sugumaran, William E Robinson
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·A C RigbyJ Stenflo
May 15, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Manickam Sugumaran, William E Robinson
Jul 3, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mingmei CaiWilliam E Robinson
Jun 3, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Geoffrey NetteMario de Vincentiis
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Jan 22, 2019·Protein and Peptide Letters·Elsie C Jimenez
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Aug 28, 2020·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Kevin H YoonAndrew J Waskiewicz
Jun 19, 2002·Analytical Biochemistry·Luis A Burzio, J Herbert Waite

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