Mos activates myogenic differentiation by promoting heterodimerization of MyoD and E12 proteins.

Molecular and Cellular Biology
J L LenormandS A Leibovitch

Abstract

The activities of myogenic basic helix-loop-helix (bHLH) factors are regulated by a number of different positive and negative signals. Extensive information has been published about the molecular mechanisms that interfere with the process of myogenic differentiation, but little is known about the positive signals. We previously showed that overexpression of rat Mos in C2C12 myoblasts increased the expression of myogenic markers whereas repression of Mos products by antisense RNAs inhibited myogenic differentiation. In the present work, our results show that the rat mos proto-oncogene activates transcriptional activity of MyoD protein. In transient transfection assays, Mos promotes transcriptional transactivation by MyoD of the muscle creatine kinase enhancer and/or a reporter gene linked to MyoD-DNA binding sites. Physical interaction between Mos and MyoD, but not with E12, is demonstrated in vivo by using the two-hybrid approach with C3H10T1/2 cells and in vitro by using the glutathione S-transferase (GST) pull-down assays. Unphosphorylated MyoD from myogenic cell lysates and/or bacterially expressed MyoD physically interacts with Mos. This interaction occurs via the helix 2 region of MyoD and a highly conserved region in Mos ...Continue Reading

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Citations

May 28, 1998·Cell Calcium·S HermannT Grundström
Oct 4, 2006·Experimental Cell Research·Sabrina Batonnet-PichonSerge A Leibovitch
Feb 13, 2001·Seminars in Cell & Developmental Biology·B Mellström, J R Naranjo
Feb 19, 2005·The EMBO Journal·Frederic LluísPura Muñoz-Cánoves
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Apr 16, 2004·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Wei-Cheng XueAnnie N Y Cheung
Nov 24, 1999·FEBS Letters·B SolhonneS A Leibovitch
Jun 6, 2000·FEBS Letters·K PelpelS A Leibovitch
Feb 27, 2018·Npj Regenerative Medicine·Nora YucelHelen M Blau
Apr 9, 2008·Physiological Reviews·Britt MellströmJose R Naranjo
Apr 15, 2000·The Journal of Biological Chemistry·E G ReynaudS A Leibovitch
Jul 11, 1998·The Journal of Biological Chemistry·E R PriceD E Fisher
Jun 21, 2001·Molecular and Cellular Biology·S BenkhelifaM P Felder-Schmittbuhl
Oct 19, 1999·Molecular and Cellular Biology·E G ReynaudS A Leibovitch
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