PMID: 11906919Mar 22, 2002Paper

Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts

Biology of Reproduction
K W ParkR S Prather

Abstract

Genetically modified domestic animals have many potential applications ranging from basic research to production agriculture. One of the goals in transgenic animal production schemes is to reliably predict the expression pattern of the foreign gene. Establishing a method to screen genetically modified embryos for transgene expression before transfer to surrogates may improve the likelihood of producing offspring with the desired expression pattern. In order to determine how transgene expression may be regulated in the early embryo, we generated porcine embryos from two distinct genetically modified cell lines by using the nuclear transfer (NT) technique. Both cell lines expressed the enhanced green fluorescent protein (eGFP); the first was a fibroblast cell line derived from the skin of a newborn pig that expressed eGFP, whereas the second was a fetal derived fibroblast cell line into which the eGFP gene was introduced by a retroviral vector. The reconstructed embryos were activated by electrical pulses and cultured in NCSU23. Although the in vitro developmental ability of each group of NT embryos was not different, the eGFP expression pattern was different. All embryos produced from the transduced fetal cell line fluoresced, b...Continue Reading

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Citations

Aug 31, 2010·Transgenic Research·Jannik E JakobsenJacob Giehm Mikkelsen
Oct 9, 2002·Journal of Virological Methods·Takele ArgawCarolyn A Wilson
Feb 10, 2011·Zygote : the Biology of Gametes and Early Embryos·So Gun HongByeong Chun Lee
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Aug 13, 2002·Cloning and Stem Cells·D Bart CarterRandall S Prather
Aug 24, 2010·Cellular Reprogramming·Joonghoon ParkXiuchun Cindy Tian
Oct 3, 2007·Cloning and Stem Cells·Nadine HornenHeiner Niemann
Mar 28, 2007·Cloning and Stem Cells·Le JiangX Cindy Tian
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Jul 13, 2012·Zygote : the Biology of Gametes and Early Embryos·Zubing CaoQiuyan Li

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