Mosaicicm in bcr-abl protein expression in B cells in chronic myelogenous leukemia

International Journal of Cancer. Journal International Du Cancer
E M WeissingerH J Kolb

Abstract

Chronic myelogenous leukemia is a disease of the pluripotent stem cell that involves the myeloid and, to a varying degree, the lymphoid compartment. We studied the involvement of B cells in chronic myelogenous leukemia at diagnosis and during treatment. B lymphocytes were immortalized by infection with Epstein-Barr virus. B-lymphoid cell lines could be established from 25 patients suffering from Philadelphia-chromosome (Ph1)-positive chronic myelogenous leukemia. The cell lines were tested for expression of the typical 210-kDa fusion protein, p210, using Western-blot analysis, and/or for mRNA expression of bcr-abl fusion genes, using reverse transcriptase polymerase chain reaction analysis. At diagnosis, mosaicism of B cells was demonstrated in every patient. During treatment with interferon alpha, p210-expressing B-lymphoid cell lines could not be established from 8 of 8 patients. Following discontinuation of IFN-alpha therapy, p210-positive cell lines were found early, even before cytogenetic recurrence. Resistance to IFN-alpha therapy and progression of the disease were both associated with the appearance of p210-positive cell lines. Cell lines established from 3 healthy individuals and from patients suffering from Ph1-negat...Continue Reading

References

Feb 1, 1991·American Journal of Hematology·S H AdvaniC N Nair
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E S KawasakiF P McCormick
Oct 15, 1988·Cancer Genetics and Cytogenetics·M E WeinsteinS E Waintraub
Mar 1, 1988·The Journal of Experimental Medicine·D Gray
Jan 1, 1983·Medical Microbiology and Immunology·M von Knebel DoeberitzH zur Hausen
Jan 12, 1994·Journal of Immunological Methods·E M WeissingerJ F Mushinski

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