Mosquito bottlenecks alter viral mutant swarm in a tissue and time-dependent manner with contraction and expansion of variant positions and diversity

Virus Evolution
Edward I PattersonNaomi L Forrester

Abstract

Viral diversity is theorized to play a significant role during virus infections, particularly for arthropod-borne viruses (arboviruses) that must infect both vertebrate and invertebrate hosts. To determine how viral diversity influences mosquito infection and dissemination Culex taeniopus mosquitoes were infected with the Venezuelan equine encephalitis virus endemic strain 68U201. Bodies and legs/wings of the mosquitoes were collected individually and subjected to multi-parallel sequencing. Virus sequence diversity was calculated for each tissue. Greater diversity was seen in mosquitoes with successful dissemination versus those with no dissemination. Diversity across time revealed that bottlenecks influence diversity following dissemination to the legs/wings, but levels of diversity are restored by Day 12 post-dissemination. Specific minority variants were repeatedly identified across the mosquito cohort, some in nearly every tissue and time point, suggesting that certain variants are important in mosquito infection and dissemination. This study demonstrates that the interaction between the mosquito and the virus results in changes in diversity and the mutational spectrum and may be essential for successful transition of the b...Continue Reading

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Citations

Aug 15, 2019·PLoS Neglected Tropical Diseases·Lucie DanetNolwenn Jouvenet
May 21, 2020·Viruses·Edward I PattersonNaomi L Forrester
Jan 23, 2019·Frontiers in Cellular and Infection Microbiology·Marine J Petit, Priya S Shah
Nov 18, 2020·Nature Communications·Lucy de Guilhem de LatailladePei-Shi Yen
Jan 13, 2021·Nature Reviews. Microbiology·Scott C WeaverNikos Vasilakis

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