Mosquito excreta: A sample type with many potential applications for the investigation of Ross River virus and West Nile virus ecology

PLoS Neglected Tropical Diseases
Ana L RamírezAndrew F van den Hurk

Abstract

Emerging and re-emerging arthropod-borne viruses (arboviruses) cause human and animal disease globally. Field and laboratory investigation of mosquito-borne arboviruses requires analysis of mosquito samples, either individually, in pools, or a body component, or secretion such as saliva. We assessed the applicability of mosquito excreta as a sample type that could be utilized during studies of Ross River and West Nile viruses, which could be applied to the study of other arboviruses. Mosquitoes were fed separate blood meals spiked with Ross River virus and West Nile virus. Excreta was collected daily by swabbing the bottom of containers containing batches and individual mosquitoes at different time points. The samples were analyzed by real-time RT-PCR or cell culture enzyme immunoassay. Viral RNA in excreta from batches of mosquitoes was detected continuously from day 2 to day 15 post feeding. Viral RNA was detected in excreta from at least one individual mosquito at all timepoints, with 64% and 27% of samples positive for RRV and WNV, respectively. Excretion of viral RNA was correlated with viral dissemination in the mosquito. The proportion of positive excreta samples was higher than the proportion of positive saliva samples,...Continue Reading

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Citations

Feb 23, 2019·Tropical Medicine and Infectious Disease·Andrew F van den HurkScott A Ritchie
Apr 3, 2019·Journal of Medical Entomology·Ana L RamírezAndrew F van den Hurk
Apr 5, 2019·Journal of Medical Entomology·Dagmar B MeyerScott A Ritchie
Apr 9, 2020·PLoS Neglected Tropical Diseases·Corrado MinettiLisa J Reimer
May 28, 2019·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Nina KuruczCheryl A Johansen
Sep 11, 2020·MSphere·Ana L RamírezAndrew F van den Hurk
Jan 23, 2022·Journal of Medical Entomology·Stephen A PeinadoLyric C Bartholomay

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Methods Mentioned

BETA
genotyping
excreta

Software Mentioned

GraphPad Prism

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