Mosquitocidal activity of the CryIC delta-endotoxin from Bacillus thuringiensis subsp. aizawai.

Applied and Environmental Microbiology
G P SmithD J Ellar

Abstract

The cloned 135-kDa CryIC delta-endotoxin from Bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against Spodoptera litoralis and Spodoptera frugiperda larvae and in vitro against the S. frugiperda Sf9 cell line. Here, we report that the CryIC delta-endotoxin cloned from B. thuringienesis subsp. aizawai HD-229 and expressed in an acrystalliferous B. thuringiensis strain is also toxic to Aedes aegypti, Anophles gambiae, and Culex quinquefasciatus mosquito larvae. Furthermore, when solubilized and proteolytically activated by insect gut extracts, CryIC is cytotoxic to cell lines derived from the first two of these dipteran insects. This activity was not observed for two other lepidopteran-active delta-endotoxins, CryIA(a) and CryIA(c). However, in contrast to the case with a lepidopteran and dipteran delta-endotoxin cloned from B. thuringiensis subsp. aizawai IC1 (M.Z. Haider, B. H. Knowles, and D. J. Ellar, Eur. J. Biochem. 156:531-540, 1986), no differences in the in vitro specificity or processing of CryIC were found when it was activated by lepidopteran or dipteran gut extract. The recombinant CryIC delta-endotoxin expressed in Escherichia coli was also toxic to A. aegypti larvae. By contr...Continue Reading

References

Jan 1, 1990·DNA Sequence : the Journal of DNA Sequencing and Mapping·T C Hodgman, D J Ellar
Jun 1, 1989·Microbiological Reviews·H Höfte, H R Whiteley
Jan 1, 1983·Drug and Chemical Toxicology·H R Lieberman
Jul 15, 1981·The Biochemical Journal·G S StewartD J Ellar

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Citations

Mar 7, 2003·Bioscience, Biotechnology, and Biochemistry·Shinya KamauchiHiroshi Sakai
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean
Apr 7, 2005·Journal of Molecular Biology·Panadda BoonsermJade Li

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