Mössbauer characterization of an unusual high-spin side-on peroxo-Fe3+ species in the active site of superoxide reductase from Desulfoarculus Baarsii. Density functional calculations on related models

Biochemistry
Olivier HornerJean-Marc Latour

Abstract

Superoxide reductase (SOR) is an Fe protein that catalyzes the reduction of superoxide to give H(2)O(2). Recently, the mutation of the Glu47 residue into alanine (E47A) in the active site of SOR from Desulfoarculus baarsii has allowed the stabilization of an iron-peroxo species when quickly reacted with H(2)O(2) [Mathé et al. (2002) J. Am. Chem. Soc. 124, 4966-4967]. To further investigate this non-heme peroxo-iron species, we have carried out a Mössbauer study of the (57)Fe-enriched E47A SOR from D. baarsii reacted quickly with H(2)O(2). Considering the Mössbauer data, we conclude, in conjunction with the other spectroscopic data available and with the results of density functional calculations on related models, that this species corresponds to a high-spin side-on peroxo-Fe(3+) complex. This is one of the first examples of such a species in a biological system for which Mössbauer parameters are now available: delta(/Fe) = 0.54 (1) mm/s, DeltaE(Q) = -0.80 (5) mm/s, and the asymmetry parameter eta = 0.60 (5) mm/s. The Mössbauer and spin Hamiltonian parameters have been evaluated on a model from the side-on peroxo complex (model 2) issued from the oxidized iron center in SOR from Pyrococcus furiosus, for which structural data ar...Continue Reading

References

Sep 15, 1988·Physical Review A: General Physics·A D Becke
May 30, 2001·FEBS Letters·V NivièreC Houée-Levin
Dec 6, 2001·Journal of the American Chemical Society·T LovellL Noodleman
May 2, 2002·Journal of the American Chemical Society·Christelle MathéVincent Nivière

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Citations

Sep 16, 2010·Inorganic Chemistry·Jay StasserDavid P Goldberg
Nov 9, 2006·Journal of the American Chemical Society·Terutaka KitagawaJulie A Kovacs
Aug 13, 2011·Journal of the American Chemical Society·Eric D BlochJeffrey R Long
Dec 17, 2009·Journal of the American Chemical Society·Frances NamusweDavid P Goldberg
May 18, 2011·BMC Microbiology·Céline Lucchetti-MiganehFrédérique Barloy-Hubler
Oct 7, 2014·Chemical Communications : Chem Comm·S RatV Nivière
Nov 9, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Amr Ali Ahmed Ali AttiaRadu Silaghi-Dumitrescu
Jul 25, 2009·Journal of Molecular Graphics & Modelling·Radu Silaghi-Dumitrescu
Mar 1, 2006·Journal of Inorganic Biochemistry·Donald M Kurtz
Jul 16, 2019·Chemical Communications : Chem Comm·Marius Horch
Jun 5, 2007·The Journal of Biological Chemistry·Christelle MathéVincent Nivière
Apr 2, 2014·Chemical Reviews·Yuewei ShengJoan Selverstone Valentine
Mar 21, 2007·Journal of the American Chemical Society·Katharina DürrIvana Ivanović-Burmazović
Nov 25, 2005·Journal of the American Chemical Society·Christelle MathéTony A Mattioli

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