PMID: 2496198May 1, 1989Paper

Most synaptic vesicles isolated from rat brain carry three membrane proteins, SV2, synaptophysin, and p65

Journal of Neurochemistry
E Floor, B E Feist

Abstract

We have prepared highly purified synaptic vesicles from rat brain by subjecting vesicles purified by our previous method to a further fractionation step, i.e., equilibrium centrifugation on a Ficoll gradient. Monoclonal antibodies to three membrane proteins enriched in synaptic vesicles--SV2, synaptophysin, and p65--each were able to immunoprecipitate specifically approximately 90% of the total membrane protein from Ficoll-purified synaptic vesicle preparations. Anti-SV2 precipitated 96% of protein, anti-synaptophysin 92%, and anti-p65 83%. These results demonstrate two points: (1) Ficoll-purified synaptic vesicles appear to be greater than 90% pure, i.e., less than 10% of membranes in the preparation do not carry synaptic vesicle-associated proteins. These very pure synaptic vesicles may be useful for direct biochemical analyses of mammalian synaptic vesicle composition and function. (2) SV2, synaptophysin, and p65 coexist on most rat brain synaptic vesicles. This result suggests that the functions of these proteins are common to most brain synaptic vesicles. However, if SV2, synaptophysin, or p65 is involved in synaptic vesicle dynamics, e.g., in vesicle trafficking or exocytosis, separate cellular systems are very likely req...Continue Reading

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Citations

May 26, 1999·The Journal of Comparative Neurology·K RichterE D Gundelfinger
Jan 15, 1992·Biological Psychiatry·W G HonerP Davies
Dec 1, 1994·Neurochemistry International·I LlonaW P De Potter
Oct 24, 1994·Neuroscience Letters·Y Wang, E Floor
Jul 4, 2001·Neurochemistry International·M W FleckA M Palmer
Aug 30, 1996·Neuroscience Letters·E Floor, L Meng
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S M BajjaliehR H Scheller
Apr 25, 2009·PloS One·Jia Yao, Sandra M Bajjalieh
Jun 1, 1991·Neuron·B WendlandR H Scheller
Jan 30, 2008·Current Protocols in Cell Biology·Branch CraigeVictor Faundez
Sep 25, 2002·The Journal of Comparative Neurology·Ilyas SingecBenedikt Volk

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