MotAB-like machinery drives the movement of MreB filaments during bacterial gliding motility

Proceedings of the National Academy of Sciences of the United States of America
Guo FuBeiyan Nan

Abstract

MreB is a bacterial actin that is important for cell shape and cell wall biosynthesis in many bacterial species. MreB also plays crucial roles in Myxococcus xanthus gliding motility, but the underlying mechanism remains unknown. Here we tracked the dynamics of single MreB particles in M. xanthus using single-particle tracking photoactivated localization microscopy. We found that a subpopulation of MreB particles moves rapidly along helical trajectories, similar to the movements of the MotAB-like gliding motors. The rapid MreB motion was stalled in the mutants that carried truncated gliding motors. Remarkably, M. xanthus MreB moves one to two orders of magnitude faster than its homologs that move along with the cell wall synthesis machinery in Bacillus subtilis and Escherichia coli, and this rapid movement was not affected by the inhibitors of cell wall biosynthesis. Our results show that in M. xanthus, MreB provides a scaffold for the gliding motors while the gliding machinery drives the movement of MreB filaments, analogous to the interdependent movements of myosin motors and actin in eukaryotic cells.

References

Dec 24, 1997·Journal of Bacteriology·S S Wu, D Kaiser
Dec 29, 1998·Journal of Bacteriology·M B ElowitzS Leibler
Mar 5, 1999·Cellular and Molecular Life Sciences : CMLS·T OkaY Natori
Sep 7, 2001·Nature·F van den EntJ Löwe
Jul 24, 2004·EMBO Reports·Hervé Joël Defeu Soufo, Peter L Graumann
Jul 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·So Yeon KimW E Moerner
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Feb 10, 2007·Science·Tâm MignotDavid R Zusman
Jan 8, 2008·Nature Methods·Makio TokunagaKumiko Sakata-Sogawa
Dec 8, 2009·Science·Thomas D Pollard, John A Cooper
May 22, 2010·Molecular Microbiology·Beiyan NanDavid R Zusman
Jul 16, 2010·Cold Spring Harbor Perspectives in Biology·Joshua W Shaevitz, Zemer Gitai
Nov 5, 2010·Annual Review of Genetics·Matthew T Cabeen, Christine Jacobs-Wagner
Jan 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Beiyan NanDavid R Zusman
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mingzhai SunTâm Mignot
Jun 4, 2011·Science·Julia Domínguez-EscobarRut Carballido-López
Aug 6, 2011·Molecular Cell·Jeanne SaljeJan Löwe
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sven van TeeffelenZemer Gitai
Sep 14, 2011·Annual Review of Genetics·Beiyan Nan, David R Zusman
Sep 21, 2011·PLoS Genetics·Jennifer LucianoTâm Mignot
Jan 31, 2012·Applied and Environmental Microbiology·Nuria Gómez-SantosJosé Muñoz-Dorado
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Siyuan WangJoshua W Shaevitz
Sep 6, 2012·Biophysical Journal·Nilah MonnierMark Bathe
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Beiyan NanDavid R Zusman
Feb 17, 2012·ACS Synthetic Biology·Yusuke T MaedaAlbert Libchaber
Jun 21, 2013·Molecular Biology of the Cell·Christian ReimoldPeter L Graumann
Sep 10, 2013·Biophysical Journal·Philipp V OlshausenAlexander Rohrbach
Feb 22, 2014·Current Biology : CB·Beiyan NanGeorge Oster
May 21, 2014·ELife·Fusinita van den EntJan Löwe
Jan 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Beiyan NanDavid R Zusman
Jul 15, 2015·The Journal of Cell Biology·Anke Treuner-LangeTâm Mignot
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Randy M MorgensteinZemer Gitai
Mar 31, 2016·Molecular Microbiology·Beiyan Nan, David R Zusman
Oct 25, 2016·Nature Communications·Timothy K LeeKerwyn Casey Huang
Nov 12, 2017·Molecular Microbiology·Connor B PogueBeiyan Nan

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Citations

Nov 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Joël TchoufagKranthi K Mandadapu
Jan 31, 2020·Frontiers in Chemistry·Elvis Awuni
May 29, 2019·Annual Review of Microbiology·Sundharraman Subramanian, Daniel B Kearns
Feb 26, 2019·Microbial Biotechnology·Grégory BoëlAntoine Danchin
Jun 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Huan ZhangBeiyan Nan
Aug 9, 2020·Frontiers in Molecular Biosciences·Divya Singhi, Preeti Srivastava
Oct 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Romain MercierTâm Mignot
May 31, 2018·Chemical Reviews·David A DikShahriar Mobashery
Sep 23, 2021·Nature Reviews. Microbiology·Navish Wadhwa, Howard C Berg

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