MotD of Sinorhizobium meliloti and related alpha-proteobacteria is the flagellar-hook-length regulator and therefore reassigned as FliK

Journal of Bacteriology
Elke EggenhoferB Scharf

Abstract

The flagella of the soil bacterium Sinorhizobium meliloti differ from the enterobacterial paradigm in the complex filament structure and modulation of the flagellar rotary speed. The mode of motility control in S. meliloti has a molecular corollary in two novel periplasmic motility proteins, MotC and MotE, that are present in addition to the ubiquitous MotA/MotB energizing proton channel. A fifth motility gene is located in the mot operon downstream of the motB and motC genes. Its gene product was originally designated MotD, a cytoplasmic motility protein having an unknown function. We report here reassignment of MotD as FliK, the regulator of flagellar hook length. The FliK gene is one of the few flagellar genes not annotated in the contiguous flagellar regulon of S. meliloti. Characteristic for its class, the 475-residue FliK protein contains a conserved, compactly folded Flg hook domain in its carboxy-terminal region. Deletion of fliK leads to formation of prolonged flagellar hooks (polyhooks) with missing filament structures. Extragenic suppressor mutations all mapped in the cytoplasmic region of the transmembrane export protein FlhB and restored assembly of a flagellar filament, and thus motility, in the presence of polyho...Continue Reading

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Citations

Oct 10, 2008·Molecular Plant-microbe Interactions : MPMI·Christelle BahlawaneAnke Becker
Aug 12, 2009·Applied and Environmental Microbiology·Rachael N Slightom, Alison Buchan
Jul 21, 2009·Journal of Bacteriology·Veronika M Meier, Birgit E Scharf
Sep 19, 2006·Journal of Bacteriology·Christine RotterBirgit Scharf
Jun 28, 2008·PLoS Pathogens·Christian LorenzDaniela Büttner
Jan 9, 2016·Biophysical Journal·Nicholas A LicataSima Setayeshgar
Mar 25, 2014·Applied and Environmental Microbiology·Benjamin A WebbBirgit E Scharf
Apr 26, 2007·Protein Science : a Publication of the Protein Society·Richard C WatersKieran A Ryan
Jun 1, 2010·Environmental Microbiology Reports·José Juan Rodríguez-HervaJuan-Luis Ramos
Nov 14, 2019·Molecular Plant Pathology·Maxuel O AndradeNian Wang

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