Mother-Specific Signature in the Maternal Transcriptome Composition of Mature, Unfertilized Zebrafish Eggs

PloS One
Han RauwerdaTimo M Breit

Abstract

Maternal mRNA present in mature oocytes plays an important role in the proper development of the early embryo. As the composition of the maternal transcriptome in general has been studied with pooled mature eggs, potential differences between individual eggs are unknown. Here we present a transcriptome study on individual zebrafish eggs from clutches of five mothers in which we focus on the differences in maternal mRNA abundance per gene between and within clutches. To minimize technical interference, we used mature, unfertilized eggs from siblings. About half of the number of analyzed genes was found to be expressed as maternal RNA. The expressed and non-expressed genes showed that maternal mRNA accumulation is a non-random process, as it is related to specific biological pathways and processes relevant in early embryogenesis. Moreover, it turned out that overall the composition of the maternal transcriptome is tightly regulated as about half of the expressed genes display a less than twofold expression range between the observed minimum and maximum expression values of a gene in the experiment. Even more, the maximum gene-expression difference within clutches is for 88% of the expressed genes lower than twofold. This means th...Continue Reading

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Citations

Jun 22, 2017·Biochemical and Biophysical Research Communications·Chunmei GeAqin Chen
Feb 9, 2018·Current Environmental Health Reports·Karilyn E Sant, Alicia R Timme-Laragy
Sep 22, 2019·Scientific Reports·Jennifer A FitzgeraldColette M Vom Berg
Nov 29, 2020·Molecular and Cellular Endocrinology·Giampaolo TrivellinConstantine A Stratakis
Sep 12, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sakshi ShardaBarbara Taborsky

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Datasets Mentioned

BETA
GPL15180
GPL
10042

Methods Mentioned

BETA
Feature Extraction
PCA

Software Mentioned

DAVID Bioinformatics Resources
Bioconductor
R
maanova
R Bioconductor maanova
R Bioconductor qvalue package
Ensembl
MATCH
Ensembl Biomart
affy

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