Motile events in pancreatic endocrine cells

Endocrinology
G SomersW J Malaisse

Abstract

Monolayer cultures of neonatal rat endocrine pancreatic cells were examined by time lapse cinematography. Motion analysis revealed two major types of motile events. On one hand, particles (0.3 micrometers in diameter) tentatively identified as secretory granules were found to undergo back and forth saltatory movements along oriented pathways. The existence of such pathways was statistically validated by the large prevalence of angles close to either 0 degrees of 180 degrees between successive movements of the same particle. The movements occurred at a mean speed of 0.8 micrometers/sec. Glucose (16.7 mM) increased the frequency of such movements. Vincristine (10 microM) caused a progressive inhibition of saltatory movements, which may depend, therefore, on the integrity of the microtubular apparatus. On the other hand, areas of the cell boundary displayed contractile-like movements, which were stimulated by insulinotropic agents, such as glucose and the ionophore A23187. Cytochalasin B also affected this second type of motile event, which is thought to reflect the activity of actin-like microfilaments. These findings suggest that the microtubular apparatus serves as a guiding cytoskeleton for the oriented translocation of secret...Continue Reading

Citations

Dec 1, 1980·Pflügers Archiv : European journal of physiology·G SomersW J Malaisse
Apr 18, 1983·Life Sciences·W Y FujimotoY Kanazawa
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·R Hoo-ParisB Sutter
Jul 1, 1984·Molecular and Cellular Endocrinology·A SenerW J Malaisse
Feb 11, 1991·Neuroscience Letters·S TerakawaM Ohara-Imaizumi
Nov 8, 1983·Biochimica Et Biophysica Acta·R GomisW J Malaisse
Aug 24, 2013·Experimental & Molecular Medicine·Michael A Kalwat, Debbie C Thurmond
Dec 6, 2011·The Journal of Biological Chemistry·Dieter RondasPhilippe A Halban
Feb 21, 2008·The Journal of Biological Chemistry·Jenna L JewellDebbie C Thurmond
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Patrick E MacDonaldPatrik Rorsman
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Li MaLouis H Philipson
Oct 15, 1984·Experientia·S L Howell, M Tyhurst
Dec 14, 2012·Kidney International·Dominik SteppanArmin Kurtz
Oct 29, 2011·Cell Biochemistry and Function·Denise de Fátima I SouzaMárcia Queiroz Latorraca
Apr 1, 1979·Metabolism: Clinical and Experimental·W J MalaisseJ C Hutton
Jan 1, 1986·Diabetes/metabolism Reviews·S L Howell, M Tyhurst
Jul 1, 1984·Cell Biochemistry and Function·R GomisW J Malaisse
Apr 7, 2006·Molecular and Cellular Endocrinology·Gabriel F AnhêSilvana Bordin
Aug 26, 1999·Japanese Journal of Pharmacology·I Niki
Nov 13, 2004·The Journal of Biological Chemistry·Angela K Nevins, Debbie C Thurmond
Apr 28, 1978·Annals of the New York Academy of Sciences·W J MalaisseE Rojas
Dec 24, 2010·American Journal of Physiology. Cell Physiology·Nizar I MouradJean-Claude Henquin
Aug 20, 2015·American Journal of Physiology. Endocrinology and Metabolism·Caroline Arous, Philippe A Halban
Sep 26, 2000·American Journal of Physiology. Cell Physiology·Y IshiharaS Terakawa
Mar 7, 2021·International Journal of Molecular Sciences·Diti Chatterjee BhowmickDebbie C Thurmond
May 1, 1982·The American Journal of Physiology·F K GorusD G Pipeleers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.