PMID: 8969069Nov 1, 1996Paper

Motility and cytoskeleton of isolated vestibular hair cells of the guinea pig

Nihon Jibiinkoka Gakkai kaiho
H Miyawaki

Abstract

Vestibular hair cells were isolated from the vestibular endorgan of the guinea pig by enzymatic and mechanical dissociation. The isolated cells were divided into three types: flask-shaped type I cells, rod-shaped type II cells and round supporting cells. The cilia of type I cells in the crista were longer than those in the corresponding cell type in the macula. After exposure to a medium containing a high concentration of potassium, to a hypoosmotic (280mOsm) medium or to medium containing ATP, the type I cells showed tilting of the neck portion accompanied by tilting of the hair bundle. Isolated living type I vestibular cells are capable of self-movement. Actin and the other proteins associated with the cytoskeleton are thought to be related to vestibular hair cell motility. The location of F-actin in the isolated vestibular hair cell was studied by using FITC-labeled phalloidin. In hair cells fixed by paraformaldehyde, actin filaments found to be located in the hair bundle and cuticular plate. In frozen-fixed cells perfused by artificial perilymph, the filaments were heavily located in the hair bundle and cuticular plate as well as throughout the cytoplasm. In frozen-fixed cells perfused with artificial endolymph containing a...Continue Reading

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