Motion clustering for deblurring multispectral optoacoustic tomography images of the mouse heart

Journal of Biomedical Optics
Adrian TaruttisVasilis Ntziachristos

Abstract

Cardiac imaging in small animals is a valuable tool in basic biological research and drug discovery for cardiovascular disease. Multispectral optoacoustic tomography (MSOT) represents an emerging imaging modality capable of visualizing specific tissue chromophores at high resolution and deep in tissues in vivo by separating their spectral signatures. Whereas single-wavelength images can be acquired by multielement ultrasound detection in real-time imaging, using multiple wavelengths at separate times can lead to image blurring due to motion during acquisition. Therefore, MSOT imaging of the heart results in degraded resolution because of the heartbeat. In this work, we applied a clustering algorithm, k-means, to automatically separate a sequence of single-pulse images at multiple excitation wavelengths into clusters corresponding to different stages of the cardiac cycle. We then performed spectral unmixing on each cluster to obtain images of tissue intrinsic chromophores at different cardiac stages, showing reduced sensitivity to motion compared to signal averaging without clustering. We found that myocardium images of improved resolution and contrast can be achieved using MSOT motion clustering correction. The correction metho...Continue Reading

References

Sep 11, 2003·Molecular Imaging·Robert A KrugerKathy D Miller
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Oct 30, 2008·Optics Express·R J ZempL V Wang
Jan 12, 2010·Journal of Biomedical Optics·Hans-Peter BrechtAlexander A Oraevsky
Apr 15, 2010·Chemical Reviews·Vasilis Ntziachristos, Daniel Razansky
Jul 17, 2010·Optics Letters·Andreas BuehlerVasilis Ntziachristos
Sep 15, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Athanasios SarantopoulosVasilis Ntziachristos

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Citations

Mar 1, 2013·Photoacoustics·Adrian TaruttisVasilis Ntziachristos
Apr 7, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Mariana VarnaEmmanuel Fort
Jun 1, 2018·Journal of Biomedical Optics·Devin O'KellyRalph P Mason
Jan 14, 2016·Nanoscale·Lei WangHao Wang
May 2, 2013·Optics Letters·Andreas BuehlerVasilis Ntziachristos
Oct 11, 2014·Nanoscale·Adrian TaruttisVasilis Ntziachristos
Jun 21, 2020·Nature Communications·Jacob T FriedleinKevin C Cossel
Mar 21, 2017·International Journal of Biomedical Imaging·Hamid Tayebi KhosroshahiAbolhassan Shakeri Bavil
Apr 3, 2020·Visual Computing for Industry, Biomedicine and Art·Xingxing ChenLei Xi
Sep 6, 2017·Scientific Reports·Mathias SchwarzVasilis Ntziachristos
Apr 4, 2015·Cancer Research·Adrian TaruttisVasilis Ntziachristos

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