Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice.

Journal of Cachexia, Sarcopenia and Muscle
Alba BlascoJordi Calderó

Abstract

The cellular mechanisms underlying the age-associated loss of muscle mass and function (sarcopenia) are poorly understood, hampering the development of effective treatment strategies. Here, we performed a detailed characterization of age-related pathophysiological changes in the mouse neuromuscular system. Young, adult, middle-aged, and old (1, 4, 14, and 24-30 months old, respectively) C57BL/6J mice were used. Motor behavioural and electrophysiological tests and histological and immunocytochemical procedures were carried out to simultaneously analyse structural, molecular, and functional age-related changes in distinct cellular components of the neuromuscular system. Ageing was not accompanied by a significant loss of spinal motoneurons (MNs), although a proportion (~15%) of them in old mice exhibited an abnormally dark appearance. Dark MNs were also observed in adult (~9%) and young (~4%) animals, suggesting that during ageing, some MNs undergo early deleterious changes, which may not lead to MN death. Old MNs were depleted of cholinergic and glutamatergic inputs (~40% and ~45%, respectively, P < 0.01), suggestive of age-associated alterations in MN excitability. Prominent microgliosis and astrogliosis [~93% (P < 0.001) and ~...Continue Reading

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Citations

Jan 7, 2021·Cells·Luisa GorzaMara Brancaccio
Feb 22, 2021·Ageing Research Reviews·Osvaldo DelbonoMaria Laura Messi
Aug 4, 2021·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ammir Yacoub HelouMarucia Chacur
Nov 18, 2021·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration

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Methods Mentioned

BETA
SMA
PCR

Software Mentioned

ImageJ
GraphPad Prism
AnalyzeSkeleton
Smart Video Tracking
MUNE

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