PMID: 8589456Nov 1, 1995Paper

Motor activity and mitotic spindle localization of the Drosophila kinesin-like protein KLP61F

Molecular Biology of the Cell
N R BartonL S Goldstein

Abstract

The KLP61F gene product is essential for Drosophila development. Mutations in KLP61F display a mitotic arrest phenotype caused by a failure in the proper separation of duplicated centrosomes (Heck et al., 1993). Sequence analysis of KLP61F identified it as a member of the bimC family of kinesin-like microtubule motor proteins. Here we report that KLP61F is distinct from KRP130, a kinesin-like protein recently purified from Drosophila embryos and suggested to be the product of the KLP61F gene (Cole et al., 1994). We also characterized recombinant KLP61F and found that it possesses microtubule-stimulated ATPase and microtubule translocation activities in vitro. In addition, we have used an affinity-purified, KLP61F-specific antiserum to localize native KLP61F and an epitope-tagged KLP61F fusion protein during various stages of mitosis in Drosophila syncytial blastoderm embryos. From early prophase through anaphase, KLP61F is coincident with the distribution of tubulin. Together these results confirm the existence of multiple bimC-like kinesins in Drosophila and suggest that KLP61F function is intrinsic to the mitotic spindle.

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Citations

Jan 18, 1996·Nature·A S KashinaJ M Scholey
Nov 21, 1996·Nature·A S KashinaW M Saxton
May 7, 1999·European Journal of Biochemistry·S A Endow
Apr 4, 1998·Molecular Biology of the Cell·Z Yang, L S Goldstein
Jun 17, 2005·Molecular Biology of the Cell·Gohta Goshima, Ronald D Vale
Jan 1, 1996·Annual Review of Genetics·M A Hoyt, J R Geiser
May 23, 1998·The Journal of Cell Biology·J G GindhartL S Goldstein
Mar 18, 2009·Cell Motility and the Cytoskeleton·Jonathan M Scholey
Jan 26, 2010·Methods : a Companion to Methods in Enzymology·Li Tao, Jonathan M Scholey
Jul 24, 1997·Biochimica Et Biophysica Acta·A S KashinaJ M Scholey
Mar 21, 2000·Biochimica Et Biophysica Acta·E R Hildebrandt, M A Hoyt
Dec 28, 1999·Annual Review of Cell and Developmental Biology·L S Goldstein, A V Philp
Jan 10, 2002·Genesis : the Journal of Genetics and Development·A DebecJ L Couderc
Sep 8, 2010·The EMBO Journal·Hannah MüllerBodo M H Lange
Jun 23, 1998·The Journal of Biological Chemistry·A RahmanL S Goldstein
Jan 24, 2018·Journal of Cell Science·Zhiyi LvJörg Großhans
Jul 3, 2021·International Journal of Molecular Sciences·Himanshu PandeyLarisa Gheber

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