Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation

Current Biology : CB
Adam D McPhersonRichard I Dorsky

Abstract

Postembryonic neurogenesis has been observed in several regions of the vertebrate brain, including the dentate gyrus and rostral migratory stream in mammals, and is required for normal behavior [1-3]. Recently, the hypothalamus has also been shown to undergo continuous neurogenesis as a way to mediate energy balance [4-10]. As the hypothalamus regulates multiple functional outputs, it is likely that additional behaviors may be affected by postembryonic neurogenesis in this brain structure. Here, we have identified a progenitor population in the zebrafish hypothalamus that continuously generates neurons that express tyrosine hydroxylase 2 (th2). We develop and use novel transgenic tools to characterize the lineage of th2(+) cells and demonstrate that they are dopaminergic. Through genetic ablation and optogenetic activation, we then show that th2(+) neurons modulate the initiation of swimming behavior in zebrafish larvae. Finally, we find that the generation of new th2(+) neurons following ablation correlates with restoration of normal behavior. This work thus identifies for the first time a population of dopaminergic neurons that regulates motor behavior capable of functional recovery.

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Citations

Jun 14, 2016·Journal of Neurogenetics·Eric J HorstickHarold A Burgess
Mar 16, 2017·The Journal of Comparative Neurology·Anna L XavierKei Yamamoto
Feb 7, 2018·Frontiers in Neuroanatomy·Lucy A HeapEthan K Scott
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Apr 3, 2020·Scientific Reports·Andrea MessinaGiorgio Vallortigara
Aug 20, 2016·The Journal of Biological Chemistry·Yu-Chia ChenPertti Panula
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Oct 19, 2019·ELife·Caroline Lei WeeSam Kunes
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Aug 3, 2021·Frontiers in Molecular Neuroscience·Laura Corradi, Alessandro Filosa
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Aug 9, 2020·Current Biology : CB·Carlos PantojaHerwig Baier
Oct 1, 2021·Frontiers in Neuroanatomy·Janina BorgonovoMiguel L Concha
Nov 26, 2021·Current Biology : CB·Iris OdstrcilFlorian Engert

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