Motor Recovery after Transplantation of Bone Marrow Mesenchymal Stem Cells in Rat Models of Spinal Cord Injury

Annals of Neurosciences
Durai Murugan MuniswamiGeorge Tharion

Abstract

Neuronal tissue has a limited potential to self-renew or get repaired after damage. Cell therapies using stem cells are promising approaches for the treatment of central nervous system (CNS) injuries. However, the clinical use of embryonic stem cells is limited by ethical concerns and other scientific consequences. Bone marrow mesenchymal stromal cells (BM-MSC) could represent an alternative source of stem cells for replacement therapy. Indeed, many studies have demonstrated that MSCs can give rise to neuronal cells as well as many tissue-specific cell phenotypes. Motor recovery by transplantation of bone marrow MSCs in rat models of spinal cord injury (SCI). Bone marrow was collected from the femur of albino Wistar rats. MSCs were separated using the Ficoll-Paque density gradient method and cultured in Dulbecco's Modified Eagle Medium supplemented with 20% fetal bovine serum. Cultured MSC was characterized by immunohistochemistry and flow cytometry and neuronal-induced cells were further characterized for neural markers. Cultured MSCs were transplanted into the experimentally injured spinal cord of Wistar rats. Control (injured, but without cell transplantation) and transplanted rats were followed up to 8 weeks, analyzed using...Continue Reading

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Citations

Nov 14, 2019·Cytotechnology·Nadia S MahmoudMohamed A Tantawy
Sep 26, 2020·Translational Stroke Research·Meng LuSeyed Esmaeil Khoshnam
Feb 26, 2020·Pharmaceuticals·Andreia G PinhoAntónio J Salgado
Jun 11, 2020·International Journal of Molecular Sciences·Rami Ahmad ShahrorKai-Yun Chen
Nov 29, 2020·Journal of Materials Science. Materials in Medicine·Durai Murugan MuniswamiGeetha Manivasagam
May 8, 2021·World Journal of Stem Cells·Nassem Ahamad, Brij B Singh

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