Mouse adaptation of a sub-genogroup B5 strain of human enterovirus 71 is associated with a novel lysine to glutamic acid substitution at position 244 in protein VP1

Virus Research
Zainun ZainiPeter C McMinn

Abstract

Most human enterovirus 71 (HEV71) strains infect only primates and are unable to cause clinically apparent infection in mice. Here we describe a mouse-adapted HEV71 strain that belongs to sub-genogroup B5 with increased virulence in newborn BALB/c mice. The mouse-virulent strain was initially selected by serial passage of a HEV71 clinical isolate (HEV71-B5) in Chinese hamster ovary (CHO) cells (CHO-B5), followed by serial passage in newborn mice. Virus from the fifth mouse passage was cultured twice on Vero cells and designated as MP-B5. MP-B5 induces severe disease of high mortality in newborn mice in a dose-dependent manner. Skeletal muscle is the primary site of virus replication and results in severe myositis. CHO-B5 harbours a single amino acid substitution (K(149) → I) in the VP2 capsid protein. Five additional nucleotide sequence changes were identified in MP-B5, two of which are located in the 5' UTR and the three within the open reading frame (ORF). Two of the ORF mutations resulted in deduced amino acid changes in the capsid protein VP1: S(241) → L and K(244) → E; the third ORF mutation was a synonymous C → T change at nucleotide position 6072 within the 3D polymerase gene. Infectious cDNA clone-derived mutant virus p...Continue Reading

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Citations

Mar 8, 2014·Journal of Biomedical Science·Jing-Yi Lin, Shin-Ru Shih
Apr 15, 2014·Current Opinion in Virology·Peng-Nien Huang, Shin-Ru Shih
Jul 17, 2013·Virus Research·Sara Sadeghipour, Peter C McMinn
Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Bing-Yu XieYi-Hong Peng
Mar 26, 2013·PloS One·Elizabeth A CaineJorge E Osorio
Mar 14, 2014·Journal of Virology·Ning DuPo Tien
Jul 19, 2013·MBio·Stefan TaubeChristiane E Wobus
Jul 25, 2019·Emerging Microbes & Infections·Liang SunHendrik Jan Thibaut
Nov 17, 2016·Scientific Reports·Issac Horng Khit TooSylvie Alonso
Oct 22, 2019·Journal of Biomedical Science·Sheng-Wen HuangJen-Ren Wang
Mar 9, 2018·Frontiers in Microbiology·Jingjing YuanLingxiang Mao
Mar 23, 2021·Emerging Microbes & Infections·Pei Yi AngSylvie Alonso
Jun 27, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Dillon Charles AdamJen Kok
Aug 29, 2021·Viruses·Kyousuke Kobayashi, Satoshi Koike

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