PMID: 3756172Oct 16, 1986Paper

Mouse ascites DNA methylase: characterisation of size, proteolytic breakdown and nucleotide recognition

Biochimica Et Biophysica Acta
R L AdamsR H Burdon

Abstract

We have purified the DNA methylase from mouse ascites tumour cells to a specific activity of 11,500 units per mg protein using denatured Micrococcus luteus DNA as methyl acceptor. Methyl groups are transferred to cytosines almost exclusively in CpG dinucleotides. The purified enzyme contains two polypeptides of molecular mass 185 and 160 kDa, and an antiserum raised in a rabbit to the purified enzyme specifically reacts with these two proteins in crude extracts. The two proteins can be partially separated by affinity chromatography when activity is associated with the 185 kDa protein which can be proteolytically degraded to give polypeptides of 170 and later 100 and 50 kDa. Only the 185 kDa methylase is lost when cells are treated with azadeoxycytidine and this is the predominant form firmly bound in the nucleus of dividing cells. Antibody bound to the 185 kDa band in protein blots will itself bind native DNA methylase, which can be detected by its binding 14C-labelled, azacytosine-containing DNA.

References

Oct 1, 1975·Nucleic Acids Research·T W SneiderL M Rogachevsky
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 27, 1979·Biochimica Et Biophysica Acta·R L AdamsR H Burdon
Jun 1, 1978·Nucleic Acids Research·D SimonH Kröger
Jun 1, 1975·European Journal of Biochemistry·D J Arndt-JovinM Marquardt
Jan 31, 1985·Biochemical and Biophysical Research Communications·T DavisR L Adams
May 24, 1985·Biochimica Et Biophysica Acta·R H BurdonR L Adams
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T H Bestor, V M Ingram
Dec 7, 1973·Biochimica Et Biophysica Acta·R L Adams, C Hogarth
Jan 21, 1969·Biochimica Et Biophysica Acta·R H Burdon, R L Adams
Aug 1, 1980·The Australian Journal of Experimental Biology and Medical Science·M TanakaT Ikeda
Jan 1, 1982·CRC Critical Reviews in Biochemistry·R L Adams, R H Burdon
Dec 15, 1982·Journal of Molecular Biology·S M Taylor, P A Jones
Apr 1, 1981·Cell·M WiglerM Perucho
Aug 2, 1983·Biochimica Et Biophysica Acta·G P PfeiferD Drahovsky
Apr 25, 1984·Nucleic Acids Research·R Y WangM Ehrlich
Sep 15, 1983·European Journal of Biochemistry·H SanoR Sager

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Citations

Mar 25, 1992·Molecular and Cellular Biochemistry·C Devajyothi, V Brahmachari
Sep 20, 1991·Journal of Molecular Biology·J E KelleherN E Murray
Jan 1, 1991·Journal of Biochemical and Biophysical Methods·R L AdamsC Seivwright
Dec 25, 1988·Gene·T H Bestor
Jun 24, 1998·Journal of Molecular Biology·J FlynnN Reich
Apr 12, 1993·FEBS Letters·R L Adams, H Lindsay
May 11, 1987·FEBS Letters·R L Adams, A Rinaldi
Oct 15, 1988·European Journal of Biochemistry·E SpiessG P Pfeifer
Jan 21, 2012·Critical Reviews in Biochemistry and Molecular Biology·Ernst G Malygin, Stanley Hattman
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R L AdamsH M Yesufu
Mar 27, 1989·FEBS Letters·R L Adams, C L So
Aug 1, 1989·Cell Biophysics·R L AdamsA Rinaldi
Mar 26, 1991·Biochimica Et Biophysica Acta·P A Hepburn, M J Tisdale
Jan 1, 1989·Archives of Biochemistry and Biophysics·G P PfeiferD Drahovsky

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