Mouse beta-globin DNA-binding protein B1 is identical to a proto-oncogene, the transcription factor Spi-1/PU.1, and is restricted in expression to hematopoietic cells and the testis.

Molecular and Cellular Biology
D L GalsonD E Housman

Abstract

The hematopoietic-specific DNA-binding protein B1 binds to the DNA consensus sequence AAAGRGGAARYG located twice in intervening sequence 2 of both of the mouse beta-globin genes (D. L. Galson and D.E. Housman, Mol. Cell. Biol. 8:381-392, 1988). B1 was cloned by expression of a murine erythroleukemia (MEL) cell cDNA library in transfected COS cells and screening by electrophoretic mobility shift analysis. B1 is identical to the proto-oncogene Spi-1/PU.1 (Spi-1), an ets family member. Protein-DNA contacts are shown to resemble those of the helix-turn-helix homeodomain proteins. By Northern (RNA) analysis, we found that Spi-1 mRNA is present at low levels during murine CFU-E maturation and is at least 20-fold higher in uninduced MEL, a transformed proerythroblast-like cell line which contains an activating/transforming insertion of spleen focus-forming virus at the Spi-1 locus. Dimethyl sulfoxide-induced MEL cell differentiation decreases Spi-1 mRNA to approximately 20% of the uninduced level before commitment occurs. In addition to erythroid cells, Spi-1 mRNA is present in B cells, myelomonocytes, and mast cells but not in T cells and nonhematopoietic cell types. In situ hybridization demonstrated Spi-1 mRNA expression in bone ma...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1978·Annual Review of Biochemistry·P A Marks, R A Rifkind
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G N WilsonR D Schmickel
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·F T KaoT T Puck
Jan 1, 1992·Annual Review of Biochemistry·C O Pabo, R T Sauer
May 1, 1992·The Journal of Experimental Medicine·C Y WangJ M Leiden
Aug 1, 1991·Genes & Development·J PelletierD Housman
Jan 1, 1992·Molecular and Cellular Biology·J M PongubalaM L Atchison
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M AffolterW J Gehring
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M SchallingM Goldstein
Jan 1, 1990·Annual Review of Biochemistry·S C Harrison, A K Aggarwal
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P SaikumarE P Reddy
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y Ben-DavidA Bernstein
Apr 1, 1990·Journal of Virology·J R ErseliusP Gruss
May 1, 1990·Human Genetics·V C NguyenF Moreau-Gachelin
Sep 11, 1990·Nucleic Acids Research·I B Dodd, J B Egan
Apr 21, 1989·Cell·A D D'AndreaG G Wong
Nov 9, 1989·Nature·J FramptonT Graf
Nov 1, 1989·Somatic Cell and Molecular Genetics·T GlaserC Jones
Jan 1, 1989·Annual Review of Biochemistry·P F Johnson, S L McKnight
Jan 1, 1989·Cytogenetics and Cell Genetics·C Junien, O W McBride
Jan 21, 1988·Nature·F Moreau-GachelinP Tambourin
May 15, 1987·Analytical Biochemistry·J K LiT Kowalik

❮ Previous
Next ❯

Citations

Jan 13, 1995·The Journal of Biological Chemistry·H SchwarzenbachP Matthias
Oct 6, 1995·The Journal of Biological Chemistry·A G RosmarinC P Simkevich
Oct 24, 1998·The Journal of Biological Chemistry·E O BockampA R Green
Nov 5, 1999·Protein Science : a Publication of the Protein Society·F PioR A Maki
Aug 9, 2003·The Journal of Biological Chemistry·Kevin M McCarthyBarbara S Nikolajczyk
Oct 15, 2005·The Journal of Biological Chemistry·James A ListmanPhilip E Auron
Jun 28, 1996·The Journal of Biological Chemistry·H ChenD G Tenen
May 20, 1998·Biotherapy·A R Migliaccio, G Migliaccio
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Eric S SilvermanWeining Lu
Jul 25, 2006·The Journal of Biological Chemistry·Chu Wai LiewJoel P Mackay
Feb 3, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Myungsoo JooJohn W Christman
Oct 16, 2009·Genes and Immunity·J L SvensonT K Nowling
Feb 25, 1998·Stem Cells·R C Fisher, E W Scott
Jun 22, 2000·Journal of Cellular Biochemistry·W Zeng, E Remold-O'Donnell
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A HuralM A Brown
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoko TodaYoshiya Tanaka
Nov 8, 2001·Blood·Y LiD G Tenen
May 25, 2005·Stem Cells and Development·Gurudutta U GangenahalliT Ravindranath
Feb 18, 2004·The Journal of Biological Chemistry·Myungsoo JooJohn W Christman
Apr 23, 2009·Journal of Cellular and Molecular Medicine·Pallavi GuptaRajendra P Tripathi
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·P ZhangZ Sun
Sep 22, 1999·Molecular and Cellular Biology·N Wara-aswapatiP E Auron
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·S ShintaniJ Klein
Jan 24, 2006·Bioscience, Biotechnology, and Biochemistry·Chiharu Nishiyama
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anser C AzimJohn W Christman

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.