PMID: 49078Jan 1, 1975Paper

Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors

Scandinavian Journal of Immunology
A B Peck, F H Bach

Abstract

We describe a miniaturized cell-mediated lympholysis assay for measuring the activity of cytotoxic lymphocytes generated in mouse mixed leukocyte cultures (MLC), using either serum-free or mouse serum-supplemented media. Mixed reactions of strain combinations possessing various genetic differences at H-2 produced cytotoxic lymphocytes with cytolytic activity against cells of the donor used in the sensitization phase. Results suggest that both serologically defined and lymphocyte-defined determinants can act as effective target antigens on target cells. In contrast, no cytotoxic lymphocytes were generated against products of non-H-2 loci--M-locus, theta, or other non-H-2 antigens--that induce MLC activation.

References

May 24, 1974·Nature·H von Boehmer, J Sprent
Dec 1, 1974·The Journal of Experimental Medicine·D J Schendel, F H Bach
May 1, 1973·The Journal of Experimental Medicine·B J AlterJ H Stimpfling
Oct 1, 1973·Journal of Immunological Methods·A B Peck, F H Bach
Jul 1, 1973·European Journal of Immunology·E Katz-HeberR E Click
Feb 1, 1972·Cellular Immunology·R E ClickB J Alter
Apr 1, 1972·The Journal of Experimental Medicine·J Klein, D C Shreffler
Mar 9, 1973·Nature·M F Caffrey, D C James

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Citations

Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B S SchepartJ A Frelinger
Mar 1, 1976·The Journal of Experimental Medicine·J W Schrader, G M Edelman
May 1, 1976·The Journal of Experimental Medicine·B J AlterF H Bach
Aug 1, 1976·The Journal of Experimental Medicine·K F Lindahl, F H Bach
Sep 1, 1978·The Journal of Experimental Medicine·F H Bach, B J Alter
Apr 1, 1980·The Journal of Experimental Medicine·A L Glasebrook, F W Fitch
Dec 1, 1981·The Journal of Experimental Medicine·S R WebbD B Wilson
Nov 1, 1982·The Journal of Experimental Medicine·S Macphail, O Stutman
Feb 1, 1976·The Journal of Allergy and Clinical Immunology·F H Bach
Mar 15, 1979·Cellular Immunology·R C Hard, R C Campbell
Mar 15, 1979·Cellular Immunology·P W WrightI D Bernstein
Jan 1, 1977·Immunological Reviews·F H BachM L Bach
Jan 1, 1980·Scandinavian Journal of Immunology·S Macphail
Feb 1, 1984·Scandinavian Journal of Immunology·R Korngold, P C Doherty
Sep 1, 1975·Scandinavian Journal of Immunology·F LindahlF H Bach
Jan 1, 1977·Scandinavian Journal of Immunology·O J Kuperman, F H Bach
Jan 1, 1977·Scandinavian Journal of Immunology·O J KupermanF H Bach
Jan 1, 1978·Scandinavian Journal of Immunology·T A Barber, B J Alter
Jan 1, 1980·Journal of Supramolecular Structure·C G OroszF H Bach
Oct 15, 1976·International Journal of Cancer. Journal International Du Cancer·R RevoltellaL Diamond
Nov 1, 1977·European Journal of Immunology·M L SoporiM L Bach
Jan 1, 1985·Critical Reviews in Clinical Laboratory Sciences·H Bril, R Benner

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