Mouse cytoplasmic dynein intermediate chains: identification of new isoforms, alternative splicing and tissue distribution of transcripts.

PloS One
Anna KutaElizabeth M C Fisher

Abstract

Intracellular transport of cargoes including organelles, vesicles, signalling molecules, protein complexes, and RNAs, is essential for normal function of eukaryotic cells. The cytoplasmic dynein complex is an important motor that moves cargos along microtubule tracks within the cell. In mammals this multiprotein complex includes dynein intermediate chains 1 and 2 which are encoded by two genes, Dync1i1 and Dync1i2. These proteins are involved in dynein cargo binding and dynein complexes with different intermediate chains bind to specific cargoes, although the mechanisms to achieve this are not known. The DYNC1I1 and DYNC1I2 proteins are translated from different splice isoforms, and specific forms of each protein are essential for the function of different dynein complexes in neurons. Here we have undertaken a systematic survey of the dynein intermediate chain splice isoforms in mouse, basing our study on mRNA expression patterns in a range of tissues, and on bioinformatics analysis of mouse, rat and human genomic and cDNA sequences. We found a complex pattern of alternative splicing of both dynein intermediate chain genes, with maximum complexity in the embryonic and adult nervous system. We have found novel transcripts, inclu...Continue Reading

References

Feb 28, 1997·The Journal of Biological Chemistry·S KarkiE L Holzbaur
Jan 9, 1998·Medical Hypotheses·M Levin, N Nascone
Jan 20, 2001·The Journal of Biological Chemistry·Y K MokM Zhang
Oct 5, 2001·Nature Cell Biology·L A LigonE L Holzbaur
Jun 22, 2002·The Journal of Biological Chemistry·Stephen J SusalkaK Kevin Pfister
May 6, 2003·Science·Majid HafezparastElizabeth M C Fisher
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Serdar E BulunMakio Shozu
Feb 27, 2004·The Journal of Biological Chemistry·Lee A LigonErika L F Holzbaur
Sep 3, 2004·Molecular Biology of the Cell·Oliver I WagnerErika L F Holzbaur
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Trina A Schroer
Nov 2, 2005·The Journal of Cell Biology·K Kevin PfisterRichard B Vallee
Jan 13, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jennifer R Levy, Erika L F Holzbaur
Jan 28, 2006·PLoS Genetics·K Kevin PfisterElizabeth M C Fisher
May 30, 2006·Biochimica Et Biophysica Acta·Erica Chevalier-Larsen, Erika L F Holzbaur
Nov 1, 2006·Journal of Cell Science·Peter Höök, Richard B Vallee
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juliane P CavistonErika L F Holzbaur
Jun 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·John C WilliamsWayne A Hendrickson
Jul 7, 2007·Molecular Cell·Andrew GrimsonDavid P Bartel
Oct 19, 2007·Nucleic Acids Research·Hiroyuki WakaguriKenta Nakai
Nov 30, 2007·Cell·Arne GennerichRonald D Vale
Apr 1, 2008·Genome Biology·Gareth T Banks, Elizabeth M C Fisher
Jun 19, 2008·The Journal of Cell Biology·Junghoon HaK Kevin Pfister
Nov 26, 2008·The Journal of Cell Biology·Jacqueline WhyteKevin T Vaughan
Dec 9, 2008·Cell Motility and the Cytoskeleton·William L TownsKevin T Vaughan
Nov 26, 2009·Nature Reviews. Molecular Cell Biology·Julia R Kardon, Ronald D Vale
Dec 31, 2009·PloS One·Séverine Mazaud GuittotRobert S Viger

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Citations

Sep 27, 2014·Nature Communications·Emmanuelle ThinonEdward W Tate
Apr 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gareth T BanksElizabeth M C Fisher
Nov 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David J MitchellK Kevin Pfister
Feb 7, 2016·Schizophrenia Research·Ary GadelhaRodrigo A Bressan
Nov 7, 2014·Neuron·Sandra MadayErika L F Holzbaur
Aug 14, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Pei Ying NgNathan J Pavlos
Apr 21, 2012·Journal of Neurochemistry·Takeshi FujiwaraHitoshi Takahashi
Sep 22, 2015·Neurobiology of Disease·Pedro Guedes-DiasJorge M A Oliveira
Sep 23, 2011·Biochemical Society Transactions·Victoria J Allan
Jul 6, 2014·The EMBO Journal·Max A SchlagerAndrew P Carter
Jul 31, 2016·The Biochemical Journal·Manasa ChanduriRashna Bhandari

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Datasets Mentioned

BETA
CB747457
GU992206
GU992207
GU992211
GU992208
GU992209
GU992212
CB784025
U39044
CF977964

Methods Mentioned

BETA
dissection
PCR
PCRs
electrophoresis
fluorescence sequencing

Software Mentioned

AliSubSimP
Ensembl Genome Browser
NanoDrop®
Target Scan Genome Browser
TargetScan
Ensembl
ClustalW2
UCSC Genome Browser

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