PMID: 9191026May 1, 1997Paper

Mouse DNA methyltransferase (MTase) deletion mutants that retain the catalytic domain display neither de novo nor maintenance methylation activity in vivo

Biological Chemistry
C ZimmermannA Graessmann

Abstract

The mammalian genome encodes a DNA cytosine-5-methyltransferase (MTase) of about 170 kDa that is apparently responsible for both de novo and maintenance methylation at CpG sites. Both methylation activities have to be regulated accurately to ensure correct developmental and cell type-specific gene activity. Distorted DNA methylation patterns have been associated with cell aging and diseases such as cancer and fragile X syndrome. Structural and functional in vitro studies of the mouse MTase have indicated that the enzyme has both a regulatory and a catalytic region located in the N-terminal and C-terminal parts of the protein, respectively. The regulatory region includes the nuclear localization signal (NLS), the sequence for DNA targeting and the Zn-binding domain. The catalytic domain carries the ten consensus sequence motifs specific for all known pro- and eukaryotic DNA cytosine-5-methyltransferases. In an attempt to separate regulatory and catalytic functions of the enzyme in vivo, we have tested various deletion mutations by means of transient and stable cell transfection experiments. Expression of the transgenes, all of which retained the C-terminal catalytic domain, was monitored by immunofluorescence staining, Northern ...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M MakosS B Baylin
Apr 1, 1992·Genetic Analysis, Techniques and Applications·T H BestorH Prydz
May 11, 1992·Nucleic Acids Research·R W YenS B Baylin
Jan 1, 1991·Journal of Biochemical and Biophysical Methods·R L AdamsC Seivwright
May 16, 1991·Nature·D FrankH Cedar
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D D DeobagkarA Graessmann
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G BuschhausenA Graessmann
Sep 1, 1984·Molecular and Cellular Biology·T H BestorV M Ingram
Jan 1, 1983·Methods in Enzymology·M Graessmann, A Graessmann
Jul 15, 1995·European Journal of Biochemistry·H TakagiA Asano
Sep 29, 1994·Nature·M BrandeisH Cedar
Jan 11, 1994·Nucleic Acids Research·S KumarG G Wilson
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T KafriA Razin
Aug 4, 1993·Journal of the National Cancer Institute·J P IssaS B Baylin

❮ Previous
Next ❯

Citations

May 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Carina Frauer, Heinrich Leonhardt
Jun 5, 2003·BMC Molecular Biology·Jean B MargotHeinrich Leonhardt
Oct 6, 1999·The Journal of Cell Biology·M C Cardoso, H Leonhardt
May 14, 2011·Biochemical and Biophysical Research Communications·Geneviève Lavoie, Yves St-Pierre
Jan 29, 2009·Journal of Cellular Biochemistry·Karin FellingerHeinrich Leonhardt
Jul 25, 2006·Advances in Enzyme Regulation·Fabio SpadaHeinrich Leonhardt
Apr 5, 2002·Chembiochem : a European Journal of Chemical Biology·Albert Jeltsch
Dec 6, 2000·The Journal of Biological Chemistry·F D AraujoM Szyf
May 20, 2009·The Biochemical Journal·Bongyong Lee, Mark T Muller
Aug 16, 2005·Biochemistry. Biokhimii︠a︡·Ya I Buryanov, T V Shevchuk
Jan 19, 2011·Chembiochem : a European Journal of Chemical Biology·Renata Zofia JurkowskaAlbert Jeltsch
Mar 29, 2002·The Journal of Biological Chemistry·Humaira Gowher, Albert Jeltsch
Aug 30, 2008·Biochemistry·Mihika PradhanSriharsa Pradhan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.