PMID: 7540599Jun 9, 1995Paper

Mouse embryo fibroblasts transformed by activated ras or dominant-negative p53 express cross-reactive tumor rejection antigens

International Journal of Cancer. Journal International Du Cancer
L J ApplemanA B Frey

Abstract

To study the immune response against oncogene-transformed tumors, C3H/HcN mouse embryo fibroblasts (MEF) were transfected with an activated allele of the H-ras proto-oncogene VaII2 and a dominant-negative allele of the murine p53 tumor suppressor gene VaII35. Transformed cell lines were derived and found to be tumorigenic in syngeneic mice. Immunization with irradiated p53 + ras-transformed MEF, but not primary MEF or unrelated syngeneic cells, protected mice from subsequent challenge with live tumor cells. The role of different immune cell subsets in the effector phase of anti-tumor immunity induced by immunization with p53 + ras-transformed MEF was investigated by in vivo antibody depletion experiments. Immunized mice depleted of CD8+ T, NK or B cells were resistant, but depletion of CD4+ T cells rendered mice susceptible to tumorigenic challenge. In contrast to the tumor-specific immune responses mounted against most chemically or UV-induced tumors, a series of independently derived p53 + ras-transformed MEF were cross-reactive in tumor rejection assays. In addition, immunization with C3H-derived L-929 cell lines expressing single gene products H-ras or p53 did not protect mice against tumorigenic challenge with p53 + ras-tr...Continue Reading

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Citations

Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S RadojaA B Frey
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S RadojaA B Frey
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S RadojaA B Frey

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