Mouse embryonic lung culture, a system to evaluate the molecular mechanisms of branching.

Journal of Visualized Experiments : JoVE
Gianni CarraroD Warburton

Abstract

Lung primordial specification as well as branching morphogenesis, and the formation of various pulmonary cell lineages requires a specific interaction of the lung endoderm with its surrounding mesenchyme and mesothelium. Lung mesenchyme has been shown to be the source of inductive signals for lung branching morphogenesis. Epithelial-mesenchymal-mesothelial interactions are also critical to embryonic lung morphogenesis. Early embryonic lung organ culture is a very useful system to study epithelial-mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceeds under specific conditions that can be readily manipulated in this system (in the absence of maternal influence and blood flow). More importantly this technique can be readily done in a serumless, chemically defined culture media. Gain and loss of function can be achieved using expressed proteins, recombinant viral vectors and/or analysis of transgenic mouse strains, antisense RNA, as well as RNA interference gene knockdown.

Citations

Feb 24, 2012·PLoS Computational Biology·Denis MenshykauDagmar Iber
Mar 19, 2016·Differentiation; Research in Biological Diversity·Kathryn S McClelland, Josephine Bowles
Feb 8, 2014·Developmental Biology·Kimberly P KeilChad M Vezina
Nov 7, 2020·International Journal of Molecular Sciences·Rajiv Kumar SahYaowu Zheng

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