Mouse EWSR1 is crucial for spermatid post-meiotic transcription and spermiogenesis.

Development
Hui Tian, Petko M Petkov

Abstract

Spermatogenesis is precisely controlled by complex gene-expression programs. During mammalian male germ-cell development, a crucial feature is the repression of transcription before spermatid elongation. Previously, we discovered that the RNA-binding protein EWSR1 plays an important role in meiotic recombination in mouse, and showed that EWSR1 is highly expressed in late meiotic cells and post-meiotic cells. Here, we used an Ewsr1 pachytene stage-specific knockout mouse model to study the roles of Ewsr1 in late meiotic prophase I and in spermatozoa maturation. We show that loss of EWSR1 in late meiotic prophase I does not affect proper meiosis completion, but does result in defective spermatid elongation and chromocenter formation in the developing germ cells. As a result, male mice lacking EWSR1 after pachynema are sterile. We found that, in Ewsr1 CKO round spermatids, transition from a meiotic gene-expression program to a post-meiotic and spermatid gene expression program related to DNA condensation is impaired, suggesting that EWSR1 plays an important role in regulation of spermiogenesis-related mRNA synthesis necessary for spermatid differentiation into mature sperm.

References

Feb 1, 1997·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A H PetersP de Boer
Nov 14, 1998·Seminars in Cell & Developmental Biology·R E Braun
May 29, 2000·The Journal of Biological Chemistry·L L Knoop, S J Baker
Apr 28, 2001·Nature Genetics·C ChoE M Eddy
Jun 11, 2004·Biology of Reproduction·Cynthia R ShirleyMarvin L Meistrich
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Igor MartianovIrwin Davidson
Aug 2, 2005·Molecular and Cellular Biology·Hiromitsu TanakaYoshitake Nishimune
Apr 4, 2006·Current Biology : CB·Satoshi H NamekawaJeannie T Lee
Apr 7, 2007·The Journal of Clinical Investigation·Hongjie LiSean Bong Lee
Sep 13, 2008·Acta Histochemica Et Cytochemica·Sadaki Yokota
Mar 14, 2009·International Journal of Andrology·Maria Paola Paronetto, Claudio Sette
Sep 25, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Liesbeth Visser, Sjoerd Repping
Sep 29, 2009·Journal of Molecular Cell Biology·Adelene Y Tan, James L Manley
Oct 27, 2009·Advances in Experimental Medicine and Biology·Rex A Hess, Luiz Renato de Franca
Dec 23, 2009·Genesis : the Journal of Genetics and Development·Amy L InselmanEdward M Eddy
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robert J A FrostEric N Olson
Jun 10, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Oliver MeikarNoora Kotaja
Aug 6, 2011·Molecular Cell·Maria Paola ParonettoJuan Valcárcel
Jan 10, 2012·Journal of Cell Science·Ippei NagamoriPaolo Sassone-Corsi
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Aug 28, 2012·The EMBO Journal·Jonathan GaucherSaadi Khochbin
Sep 13, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Allan BradleyWolfgang Wurst
Jan 5, 2013·Current Topics in Developmental Biology·Binyamin D Berkovits, Debra J Wolgemuth
Sep 17, 2013·Nucleic Acids Research·Ralf SchmidDavid J Elliott
Oct 5, 2013·Biochimica Et Biophysica Acta·Christina RathkeRenate Renkawitz-Pohl
Nov 5, 2013·Cell Death and Differentiation·K Y KimH Ryu
Jan 21, 2014·BMC Genomics·Gennady MargolinR Daniel Camerini-Otero
Feb 21, 2014·RNA·Oliver MeikarNoora Kotaja
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Apr 24, 2014·Seminars in Cell & Developmental Biology·Sara de Mateo, Paolo Sassone-Corsi
Dec 11, 2014·Annual Review of Biochemistry·Jacob C SchwartzRoy R Parker
Mar 13, 2015·Genes & Development·Anastassios VourekasP Jeremy Wang
Jun 14, 2015·Animal Reproduction Science·Rita Luiza Peruquetti
Jan 23, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Mari S Lehti, Anu Sironen
May 7, 2016·PLoS Genetics·Grigorios FanourgakisRolf Jessberger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.