Mouse glomerular epithelial cells in culture with features of podocytes in vivo express aminopeptidase A and angiotensinogen but not other components of the renin-angiotensin system.

Journal of the American Society of Nephrology : JASN
S MentzelK J Assmann

Abstract

The binding of antibodies to podocytic antigens such as the Heymann antigen or aminopeptidase A may lead to the induction of a membranous glomerulonephritis in several species. To study the possible future interactions of antibodies with antigens on these podocytes, epithelial cells from isolated mouse glomeruli were cultured. By indirect immunofluorescence, the cells were positive for cytokeratin, vimentin, desmin, and the ZO-1 protein, a component of the tight junction complex. When rat monoclonal antibodies were used, the cells were also positive for the hydrolases aminopeptidase A and dipeptidyl peptidase IV, and they stained with ASD-33, a monoclonal antibody that recognized an epitope only present on the cell membranes of mouse podocytes. They were negative for the von Willebrand factor and did not stain with a monoclonal antibody (ASD-13) that binds to endothelial cells of glomeruli and peritubular capillaries. By electron microscopy, the cells showed tight junctions but lacked Weibel Palade bodies (endothelium), desmosomes, and cilia (parietal epithelium). The mRNA expression of several components of the renin-angiotensin system was also examined, and some factors indirectly coupled to the renin-angiotensin system compo...Continue Reading

Citations

Sep 15, 1998·Kidney International. Supplement·J GloyH Pavenstädt
Jun 8, 2002·Biochemical and Biophysical Research Communications·Mamoru FujitaMasataka Majima
Apr 26, 2007·Kidney International·S J ShanklandP Mundel
Dec 10, 2008·Nature Clinical Practice. Nephrology·Juan Carlos Q Velez
Jun 12, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Soo Young YoonSeo-Hee Rha
Nov 18, 2009·Journal of the American Society of Nephrology : JASN·Bart SmeetsMarcus J Moeller
Jul 16, 2010·American Journal of Physiology. Renal Physiology·Takayuki MatsumotoJeffrey B Kopp
Mar 14, 2002·The American Journal of Pathology·Hirokazu OkadaHiromichi Suzuki

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