PMID: 6169842Sep 1, 1981Paper

Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3

Journal of Virology
M M LaiS A Stohlman

Abstract

The composition and structure of the mouse hepatitis virus (MHV)-specific RNA in actinomycin D-treated, infected L-2 cells were studied. SEven virus-specific RNA species with molecular weights of 0.6 X 10(6), 0.9 X 10(6), 1.2 X 10(6), 1.5 X 10(6), 3.0 X 10(6), 4.0 X 10(6), and 5.4 X 10(6) (equivalent to the viral genome) were detected. T1 oligonucleotide fingerprinting studies suggested that the sequences of each RNA species were totally included within the next large RNa species. The oligonucleotides of each RNA species were mapped on the 60S RNA genome of the virus. Each RNA species contained the oligonucleotides starting from the 3' end of the genome and extending continuously for various lengths in the 3' leads to 5' direction. All of the viral RNA species contained a polyadenylate stretch of 100 to 130 nucleotides and probably identical sequences immediately next to the polyadenylate. These data suggested that the virus-specific RNAs are mRNA's and have a stairlike structure similar to that of infectious bronchitis virus, an avian coronavirus. A proposal is presented, based on the mRNA structure, for the designation of the genes on the MHV genome. Using this proposal, the sequence differences between A59, a weakly pathogen...Continue Reading

References

Oct 1, 1977·Journal of Virology·B Lomniczi, I Kennedy
Oct 1, 1977·Journal of Biochemistry·Y YogoM Matumoto
May 1, 1978·Journal of Virology·M M Lai, S A Stohlman
Nov 1, 1978·The Journal of General Virology·H WegeV ter Meulen
Jan 1, 1979·Journal of Virology·J S Guy, D A Brian
Jun 1, 1978·The Journal of General Virology·M R Macnaughton, M H Madge
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Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
May 1, 1981·Journal of Virology·M M Lai, S A Stohlman
Jan 1, 1980·Journal of Virology·S G SiddellV ter Meulen

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Citations

Dec 1, 1986·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M M Lai
Jan 1, 1983·Archives of Virology·J C ChildsR Russell
Jan 1, 1983·Archives of Virology·M M LaiK Fujiwara
Jan 1, 1982·Microbiology and Immunology·F TaguchiK Fujiwara
Jun 1, 2007·Journal of Virology·Tetsuro IkegamiShinji Makino
Sep 16, 2004·Journal of Virology·Chun-Jen ChenShinji Makino
Aug 8, 2013·PloS One·Hung-Yi WuNai-Yun Chang
Oct 10, 1998·Journal of Virology·S AnS Makino
Dec 3, 2020·Nucleic Acids Research·Sylvain de BreyneThéophile Ohlmann
Jan 9, 2021·Biochemical and Biophysical Research Communications·Anastasia A Gulyaeva, Alexander E Gorbalenya
Mar 26, 2021·Cell Reports·Izumi KimuraKei Sato
Mar 1, 1986·Journal of Virology·L JacobsM C Horzinek
Mar 1, 1986·Journal of Virology·S MakinoM M Lai

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