PMID: 6020006Apr 1, 1967Paper

Mouse isoantigens: separation of soluble TL (thymus-leukemia) antigen from soluble H-2 histocompatibility antigen by column chromatography

The Journal of Experimental Medicine
D A DaviesE Stockert

Abstract

Mouse H-2 histocompatibility antigen has been extracted, solubilized, and partly purified from the cells of an A strain spontaneous leukemia carrying TL (thymus-leukemia) antigens. H-2 and TL. 1, 2, 3 activities were measured by inhibition of the cytotoxic effect of the corresponding isoantibodies. TL activity was associated with the H-2 active fraction obtained by solubilization and fractionation by gel filtration. TL specificity was largely separated from H-2 antigen by subsequent chromatography on DEAE Sephadex as an adjacent component in a series of fractions. The soluble H-2 antigen prepared from the leukemia cells was tested for most of the specificities determined by H-2(a) with no exceptional results. TL. 1, 2, 3 activities, measured as each component separately, were located in approximately the same position; there is no clear indication yet whether the three TL specificities are separable from one another. It appears that in addition to the close genetic linkage between the H-2 and TL loci, and their reciprocal interaction in producing H-2 and TL antigens, these antigens exhibit some similarity at the chemical level.

References

Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·S G Nathenson, D A Davies
Sep 1, 1964·The Journal of Experimental Medicine·A E REIF, J M ALLEN

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Citations

Jan 1, 1973·Progress in Biophysics and Molecular Biology·R C Hughes
Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·E A BoyseL J Old
Jul 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·E A BoyseE Stockert
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P A PetersonJ B Lindblom
Sep 21, 2010·Immunology Letters·Danyvid Olivares-Villagómez, Luc Van Kaer
Dec 1, 1974·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D A DaviesA J Manstone

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