PMID: 39757Aug 1, 1979

Mouse-liver glutathione reductase. Purification, kinetics, and regulation

European Journal of Biochemistry
J Lopez-Barea, C Y Lee


Glutathione reductase from the liver of DBA/2J mice was purified to homogeneity by means of ammonium sulfate fractionation and two subsequent affinity chromatography steps using 8-(6-aminohexyl)-amino-2'-phospho-adenosine diphosphoribose and N6-(6-aminohexyl)-adenosine 2',5'-biphosphate-Sephadex columns. A facile procedure for the synthesis of 8-(6-aminohexyl)-amino-2'-phospho-adenosine diphosphoribose is also presented. The purified enzyme exhibits a specific activity of 158 U/mg and an A280/A460 of 6.8. It was shown to be a dimer of Mr 105000 with a Stokes radius of 4.18 nm and an isoelectric point of 6.46. Amino acid composition revealed some similarity between the mouse and the human enzyme. Antibodies against mouse glutathione reductase were raised in rabbits and exhibited high specificity. The catalytic properties of mouse liver glutathione reductase have been studied under a variety of experimental conditions. As with the same enzyme from other sources, the kinetic data are consistent with a 'branched' mechanism. The enzyme was stabilized against thermal inactivation at 80 degrees C by GSSG and less markedly by NADP+ and GSH, but not by NADPH or FAD. Incubation of mouse glutathione reductase in the presence of NADPH or N...Continue Reading


Mar 25, 1992·Molecular and Cellular Biochemistry·J PeinadoJ Lopez-Barea
May 22, 2010·The Protein Journal·Betul CanNazmi Ozer
Jan 17, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Kavita AroraArvind K Srivastava
Jan 1, 1986·Biochemical Pharmacology·D J Reed
Jan 1, 1993·The International Journal of Biochemistry·C García-AlfonsoJ Lopez-Barea
Apr 1, 1993·The International Journal of Biochemistry·C García-AlfonsoJ Lopez-Barea
Jan 1, 1993·Biochimie·E Martínez-GalisteoJ A Bárcena
Jan 1, 1988·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M MakaryG W Ivie
Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M MakaryG W Ivie
Jan 1, 1994·Free Radical Biology & Medicine·I I Karuzina, A I Archakov
Oct 1, 1995·The International Journal of Biochemistry & Cell Biology·P VelascoR Barcia
Jan 1, 1987·Journal of Toxicology and Environmental Health·B J Buckley, D J Bassett
Oct 1, 1986·Plant Physiology·J P Connell, J E Mullet
Sep 2, 1985·European Journal of Biochemistry·M C PintoJ Lopez-Barea
Mar 30, 1990·European Journal of Biochemistry·M TuticD Werner
Jan 15, 1996·European Journal of Biochemistry·R L Krauth-SiegelR H Schirmer
Jun 11, 2009·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A A AgarkovA V Semenikhina
May 5, 2009·Free Radical Biology & Medicine·Valérie TurcotJean-Claude Lavoie
Jun 5, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Luciano A CardosoMarcelo Hermes-Lima
Sep 1, 1986·Experimental Eye Research·M C Ng, H Shichi
Aug 1, 1983·Analytical Biochemistry·N Le TrangA Cerami
Apr 15, 1986·Biochemical Pharmacology·B WermuthF Oesch
Oct 15, 2013·Free Radical Biology & Medicine·Venkat R PannalaRanjan K Dash
Jun 24, 2015·Extremophiles : Life Under Extreme Conditions·Mikyoung JiAmy M Grunden
Mar 7, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mekala Gunaratnam, M Helen Grant
Jul 9, 1999·Archives of Biochemistry and Biophysics·S DukanD Touati
Jan 1, 1984·Archives of Biochemistry and Biophysics·M C PintoJ Lopez-Barea


Dec 15, 1975·European Journal of Biochemistry·D J Worthington, M A Rosemeyer
Sep 14, 1976·Biochimica Et Biophysica Acta·K NakashimaK Yamauchi
Aug 1, 1976·European Journal of Biochemistry·D J Worthington, M A Rosemeyer
Jan 25, 1977·Biochemistry·C C Guilbert, S L Johnson
Jun 1, 1975·Archives of Biochemistry and Biophysics· Lee C-Y, N O Kaplan
Jan 15, 1977·Archives of Biochemistry and Biophysics·C Y LeeN O Kaplan
Jul 1, 1975·Archives of Biochemistry and Biophysics·A Yoshida, V Dave
Sep 9, 1977·Biochemical and Biophysical Research Communications·T OrtegaM Losada
Jun 15, 1977·Journal of Molecular Biology·H A ZappeG E Schulz
Jun 1, 1975·Biochemical Genetics·E A Nichols, F H Ruddle
Aug 21, 1973·Biochemical and Biophysical Research Communications·B Mannervik
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
Jun 17, 1969·Biochimica Et Biophysica Acta·A OmachiH Hegarty
Jul 8, 1969·Biochimica Et Biophysica Acta·G E Staal, C Veeger
Oct 27, 1970·Biochimica Et Biophysica Acta·S Hosoda, W Nakamura
Feb 9, 1973·Science·A Yoshida
May 20, 1974·Biochimica Et Biophysica Acta·I Ii, H Sakai
Oct 1, 1974·European Journal of Biochemistry·D J Worthington, M A Rosemeyer
Aug 25, 1972·Biochimica Et Biophysica Acta·A J MacGillivrayJ Paul
Mar 1, 1970·Experimental Cell Research·A Fredborg, P E Lindahl
Jul 8, 1969·Biochimica Et Biophysica Acta·G E StaalC Veeger
Oct 1, 1969·The Journal of Clinical Investigation·E Beutler
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Jun 1, 1963·The Biochemical Journal·R Nesbakken, L Eldjarn

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